GATE BT 2010 Question Paper & Solutions

Biomolecules, enzyme kinetics

a chenion growing der steady sale a bacterial culture can a grown a divin a higher

Downstream processing (chromatography, filtration)

Ames test is used to determine

Recombinant DNA technology

The bacteria known to be naturally competent for transformation of DNA is

Recombinant DNA technology

Antibiotic resistance marker that CANNOT be used in a cloning vector in Gram negative bacteria is

Biomolecules, enzyme kinetics

Program used for essentially local similarity search is

Biomolecules, enzyme kinetics

Peptidyl transferase activity resides in

Recombinant DNA technology

In transgenics, alterations in the sequence of nucleotide in genes are due to: P. Substitution Q. Deletion R. Insertion S. Rearrangement

DNA replication, transcription, translation

During transcription in prokaryotic cells, the movement of RNA polymerase along the DNA double helix produces topological changes in the template:

Biomolecules, enzyme kinetics

Under stress conditions bacteria accumulate

DNA replication, transcription, translation

An exampie for template independent DNA polymerase is

Biomolecules, enzyme kinetics

Which one of the following DOES NOT belong to the domain of Bacteria?

Biomolecules, enzyme kinetics

Interferon-beta (IFN-β) is a crucial type I interferon cytokine primarily produced by:

Probability and Statistics

A culture of bacteria is infected with bacteriophage at a multiplicity of 0.3. The probability of a single cell infected with 3 phages is

Cell structure and signaling

A neonatally thymectomized mouse. immunized with protein antigen shows

Cell structure and signaling

Lymphocytes interact with foreign antigens in

Recombinant DNA technology

Somatic cell gene transfer is used for Q. transgenic diploid cell production P. Iransgenic animal production R. in-vitro fertilization S. classical breeding of farm animals

Biomolecules, enzyme kinetics

Accession number is a unique identification assigned to a

Recombinant DNA technology

Expressed Sequence Tag is defined as

Bioreactor types, scale-up

In a chemostat growing under steady state, a bacterial culture can be grown at a dilution rate higher than the maximum specific growth rate by:

Bioenergetics and Metabolism

During lactic acid fermentation, net yield of ATP and NADH per mole of glucose is

Mass transfer in bioprocesses (kLa)

Identify the enzyme that catalyzes the following reaction a-Keloglutarate + NADH + NH, + H° ? Glutamate + NAD' + H-O

Biomolecules, enzyme kinetics

The degree of inhibition for an enzyme catalyzed reaction at a particular inhibitor concentration is independent of initial substrate concentration. The inhibition follows

Bioenergetics and Metabolism

Oxidation reduction reactions with positive standard reduction potential (ΔE°') will have

Biomolecules, enzyme kinetics

Nuclease-hypersensitive sites in the chromosomes are typically

Biomolecules, enzyme kinetics

The formation of peptide cross-links between adjacent peptidoglycan chains in bacterial cell wall synthesis is catalyzed by

Downstream processing (chromatography, filtration)

Determine the correctness or otherwise of the following Assertion [a] and Reason [r]: [a] Gram-negative bacteria are more resistant to penicillins than Gram-positive bacteria. [r] The outer membrane of Gram-negative bacteria acts as a permeability barrier to penicillins.

Biomolecules, enzyme kinetics

An immobilized enzyme being used in a continuous plug low reactor exhibits an effectiveness factor (m) of 1.2. The value of y being greater than I 0) could be apparently due to

Cell structure and signaling

A roller bottle culture vessel perfectly cylindrical in shape having inner radius (r) = 10 em and length (I) = 20 cm was fitted with a spiral film of length (L) = 30 cm and width (W) = 20 cm. If the film can support 10' anchorage dependent cells per cm', the increase in the surface area after fitting the spiral film and the additional number of cells that can be grown respectively are 1200 cm' and 12 x 10' cells

Mass transfer in bioprocesses (kLa)

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r) Assertion: formation by DNA fragmentation. MTT assay is used to determine cell viability based on the principle of colour Reason: converting tetrazolium soluble salt 10 insoluble salt MTT assay is used to determine cell viability based on ite colour development b

DNA replication, transcription, translation

Match the following antibiotics in Group I with their mode of action in Group II: Group I: P. Chloramphenicol Q. Rifampicin R. Tetracycline S. Ciprofloxacin Group II: 1. Inhibits RNA polymerase 2. Inhibits peptidyl transferase 3. Inhibits DNA gyrase 4. Inhibits aminoacyl-tRNA binding to A-site

Mass transfer in bioprocesses (kLa)

Match the chemicals in Group I with the possible type/class in Group II: Group I: P. Actinomycin D Q. Methotrexate R. 5-Fluorouracil S. Colchicine Group II: 1. Dihydrofolate reductase inhibitor 2. Intercalates into DNA and inhibits transcription 3. Microtubule polymerization inhibitor 4. Thymidylate synthase inhibitor

Recombinant DNA technology

Match Group I with Group II: Group I: P. Western blotting Q. Northern blotting R. Southern blotting S. Dot blotting Group II: 1. RNA detection 2. Protein detection 3. DNA detection 4. Rapid profiling without gel electrophoresis

DNA replication, transcription, translation

Match the promoters listed in Group I with the tissues listed in Group Il Group! Group Il P. a-Amylase Q. Glutenin 1. Endosperm 2. Tuber R. Phaseollin 3. Aleurone S. Patatin 4. Cotyledon

Recombinant DNA technology

Consider the following statements: [I] All enzymes are proteins. [II] Ribozymes are catalytic RNA molecules.

Biomolecules, enzyme kinetics

The lurnover numbers for the enzymes. El and E2 are 150 s"' and 15 s" respectively. This means

Biomolecules, enzyme kinetics

Match the items in Group I with Group II: Group I (Microorganism): P. Saccharomyces cerevisiae Q. Aspergillus niger R. Corynebacterium glutamicum S. Penicillium chrysogenum Group II (Commercial Product): 1. Citric acid 2. Ethanol 3. Penicillin 4. Glutamic acid

Biomolecules, enzyme kinetics

Match Group I with Group li Group! Group lI P. Staphylococcus aureus R. Mycobacterium tuberculosis Q. Candida albicans 1. Biofilms 3. Methicillin resistance 2. Bacteriocins S. Lacsobacillus lacsis 4. Isoniazid

Biomolecules, enzyme kinetics

A mutant Ga protein with increased GPase activity would

Cell structure and signaling

Dizygotic twins are connected to a single placenta during iheir embryonic development. These wins

Cell structure and signaling

The dissociation constant K, for ligand binding to the receptor is 10-' M. The concentration of ligand required for occupying 10% of receptors is

Cell structure and signaling

Receptor R is over expressed in CHO cells and analysed for expression. 6 x 10' cells were incubated with its radioactive ligand (specific activity 100 counts per picomole). If the total counts present in cell pellet was 1000 pm. the average number of receptors R per cell is (assume complete saturation of receptors with ligand and one ligand binds to one receptor)

Probability and Statistics

A cell has five molecules of a rare mRNA. Each cell contains 10^-12 g of total RNA, of which 2% is mRNA. The average molecular weight of an mRNA molecule is 3 × 10^5 g/mol. What fraction of total mRNA does this rare mRNA represent?

Recombinant DNA technology

Match the products in Group I with the microbial culture systems in Group II: Group I: P. Primary metabolite Q. Secondary metabolite R. Recombinant therapeutic protein S. Single cell protein Group II: 1. Trophophase synthesis 2. Idiophase synthesis 3. High-cell density fed-batch 4. Continuous culture

Classical Genetics and Epistasis

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r) Assertion: function. Cytoplasmic male sterility (cms) is invariably due to defect(s) in mitochondrial Reason: cms can be overcome by pollinating a ferility restoring (Rf) plant with pollen from a non cms plant.

Media Sterilization and Stoichiometry

Thermal death of microorganisms in liquid medium follows first order kinetics. If the initial cell concentration in the fermentation medium is 10^7 cells/ml and the final acceptable contamination level is 10^-3 cells, for how long should 1 m^3 medium be treated at 120 °C (thermal deactivation rate constant = 0.23 / min) to achieve acceptable load?

Mass transfer in bioprocesses (kLa)

True breeding Drosophila flies with curved wings and dark bodies were mated with true breeding short wings and tan body Drosophila. The F1 progeny was observed to be with curved wings and tan body. The F1 progeny was again allowed to interbreed and produced flies of following phenotypes. What is the recombination frequency between wing shape and body colour?

Downstream processing (chromatography, filtration)

Match Group I with Group II: Group I: P. Real Time-PCR Q. 2-D Electrophoresis R. Affinity chromatography S. Microarray Group II: 1. Biochips 2. SYBR Green 3. Antibody linked sepharose beads 4. Ampholytes

Bioreactor types, scale-up

The Now rate required lo result in steady state concentration of sucrose as 1.5 g / L in she bioreactor will be

Bioreactor types, scale-up

A culture of Rhizobium is grown in a chemostat (100 m^3 bioreactor). The feed contains 12 g/L sucrose, Ks for the organism is 0.2 g/L and μmax = 0.3 h^-1. If Yx/s = 0.4 g/g for the above culture and steady state concentration of sucrose is 1.5 g/L in the bioreactor, the biomass concentration will be

Downstream processing (chromatography, filtration)

The number of c-helical turns permeating the membrane is

Bioenergetics and Metabolism

The number of amino acid residues present in ibe protein is

Biomolecules, enzyme kinetics

The AG° for the oxidation of NADH by FAD is

Biomolecules, enzyme kinetics

The AG° for the oxidation of NADH by FAD is -61.8 kJ/mol. The value of ΔG, given Keq is 1.7 at 25°C, will be

Biomolecules, enzyme kinetics

The mean value of acid concentration will be

Probability and Statistics

During bioconversion of sucrose to citric acid by Aspergillus niger, final samples of 6 batches of fermentation broth were analyzed for citric acid content. The results (in g/L) were found to be 47.3, 52.2, 49.2, 52.4, 49.1 and 46.3. The standard deviation for the above results is

Verbal Aptitude

Which of the following options is the closest in meaning to the word below: Circuitous

Verbal Aptitude

The question below consists of a pair of related words followed by four pairs of words. Select she pair payed tresses the relation in the original pair.

Verbal Aptitude

Choose the most appropriate word from the options given below to complete the following sentence: If we manage to ________ our natural resources, we would leave a better planet for our children.

Verbal Aptitude

Choose she most appropriate word from the options given below to complete she following sensence: His rather casual remarks on politics his lack of seriousness about the subject

Analytical & Spatial Aptitude

Which of the following assertions about chemical warfare agents is most logically consistent with international treaties prohibiting chemical weapons?

Verbal Aptitude

Modern warfare has changed from large scale clashes of armies to suppression of civilian populations. Chemical agents that do their work silently appear to be suited to such warfare; and regretfully, there exist people in military establishments who think that chemical agents are useful. Which of the following statements best sums up the meaning of the above passage?

Quantitative Aptitude

1f 137 + 276 = 435 how much is 731 + 672?

Quantitative Aptitude

5 skilled workers can build a wall in 20 days; 8 semi-skilled workers can build a wall in 25 days; § unskilled workers. how long will it take to build the wall? 10 unskilled workers can build a wall in 30 days. If a team has 2 skilled, 6 semi-skilled and

Quantitative Aptitude

Given digits 2, 2. 3, 3, 3,4, 4. 4, 4 how many distinct 4 digit numbers greater than 3000 can be formed?

Quantitative Aptitude

Hari (H), Gita (G), Irfan (I) and Saira (S) are siblings. If H is older than G, I is younger than S, and G is older than S, what is the sequence from oldest to youngest?