Subtopic: Fundamentals of Biomolecules, enzyme kinetics
Question Statement:
An immobilized enzyme being used in a continuous plug low reactor exhibits an effectiveness
factor (m) of 1.2. The value of y being greater than I 0) could be apparently due to
Options:
(A) substrate inhibited kinetics with
(B) external pore diffusion limitation
(C) sigmoidal kinetics
internal pore diffusion limitation
(1)) unstability of the enzyme
Official Answer Key:
A
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2010 Q.27)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: Biotechnology
- **Question Type**: **MCQ** (2 Marks)
- **Core Topic**: Biochemistry, Microbiology & Molecular Biology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"An immobilized enzyme being used in a continuous plug low reactor exhibits an effectiveness
factor (m) of 1.2. The value of y being greater than I 0) could be apparently due to..."*
- **Option (A) [CORRECT]**: *"substrate inhibited kinetics with"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (B) [INCORRECT]**: *"external pore diffusion limitation"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (C) [INCORRECT]**: *"sigmoidal kinetics
internal pore diffusion limitation
(1)) unstability of t"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **A**
- **Scoring Criteria**: 2 Marks (Negative marking: -0.66 marks).
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