GATE BT 2010 • Q21 MCQ • 1 Mark

Section 4: Bioprocess Engineering and Process Biotechnology

Mass transfer in bioprocesses (kLa)

Subtopic: Fundamentals of Mass transfer in bioprocesses (kLa)

Question Statement:

Identify the enzyme that catalyzes the following reaction a-Keloglutarate + NADH + NH, + H° ? Glutamate + NAD' + H-O

Options:

  • (A) Giulamate synthetase Glutamate dchydrogenase
  • (B) Glutamate oxoglutarate aminotransferase
  • (D) a-keroglutarate deaminase

Official Answer Key:

C

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2010 Q.21)** **1. Governing Concept & Syllabus Classification**: - **Section**: Biotechnology - **Question Type**: **MCQ** (1 Mark) - **Core Topic**: Biochemistry, Microbiology & Molecular Biology **2. Detailed Step-by-Step Analytical & Scientific Derivation**: - **Systematic Conceptual & Option-by-Option Evaluation**: - Problem Focus: *"Idenify the enzyme that catalyzes the following reaction a-Keloglutarate + NADH + NH, + H° ? Glutamate + NAD' + H-O..."* - **Option (C) [CORRECT]**: *""* - Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required. - **Option (A) [INCORRECT]**: *"Giulamate synthetase Glutamate dchydrogenase"* does not satisfy the precise boundary conditions or molecular specificity. - **Option (B) [INCORRECT]**: *"Glutamate oxoglutarate aminotransferase"* does not satisfy the precise boundary conditions or molecular specificity. - **Option (D) [INCORRECT]**: *"a-keroglutarate deaminase"* does not satisfy the precise boundary conditions or molecular specificity. **3. Final Answer / Verification Summary**: - **Official GATE Answer Key**: **C** - **Scoring Criteria**: 1 Mark (Negative marking: -0.33 marks).

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