Subtopic: Fundamentals of Downstream processing (chromatography, filtration)
Question Statement:
Match Group I with Group II:
Group I:
P. Real Time-PCR
Q. 2-D Electrophoresis
R. Affinity chromatography
S. Microarray
Group II:
1. Biochips
2. SYBR Green
3. Antibody linked sepharose beads
4. Ampholytes
Options:
(A) P-2, Q-4, R-3, S-1
(B) P-1, Q-2, R-4, S-3
(C) P-2, Q-3, R-4, S-1
(D) P-3, Q-2, R-1, S-4
Official Answer Key:
A
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2010 Q.47)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: Biotechnology
- **Question Type**: **MCQ** (2 Marks)
- **Core Topic**: Biochemistry, Microbiology & Molecular Biology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"Match the techniques in Group I with their primary applications in Group II:
Group I:
P. Real Time-PCR
Q. Southern blotting
R. Western blotting
S. Northern blotting
Group II:
1. DNA fragment detection
2. Quantitative tra..."*
- **Option (C) [CORRECT]**: *"P-4, Q-3, R-2, S-1"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (A) [INCORRECT]**: *"P-2, Q-1, R-3, S-4"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (B) [INCORRECT]**: *"P-1, Q-2, R-4, S-3"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (D) [INCORRECT]**: *"P-3, Q-4, R-1, S-2"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **C**
- **Scoring Criteria**: 2 Marks (Negative marking: -0.66 marks).
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