GATE BT 2010 • Q4 MCQ • 1 Mark

Section 3: Genetics, Cellular and Molecular Biology

Recombinant DNA technology

Subtopic: Fundamentals of Recombinant DNA technology

Question Statement:

Antibiotic resistance marker that CANNOT be used in a cloning vector in Gram negative bacteria is

Options:

  • (A) Streptomycin
  • (B) Ampicillin
  • (C) Vancorycin
  • (D) Kanamycin

Official Answer Key:

C

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2010 Q.4)** **1. Governing Concept & Syllabus Classification**: - **Section**: Biotechnology - **Question Type**: **MCQ** (1 Mark) - **Core Topic**: Quantitative & Analytical Aptitude **2. Detailed Step-by-Step Analytical & Scientific Derivation**: - **Systematic Conceptual & Option-by-Option Evaluation**: - Problem Focus: *"Antibiotic resistance marker that CANNOT be used in a cloning vector in Gram negative bacteria is..."* - **Option (C) [CORRECT]**: *"Vancorycin"* - Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required. - **Option (A) [INCORRECT]**: *"Streptomycin"* does not satisfy the precise boundary conditions or molecular specificity. - **Option (B) [INCORRECT]**: *"Ampicillin"* does not satisfy the precise boundary conditions or molecular specificity. - **Option (D) [INCORRECT]**: *"Kanamycin"* does not satisfy the precise boundary conditions or molecular specificity. **3. Final Answer / Verification Summary**: - **Official GATE Answer Key**: **C** - **Scoring Criteria**: 1 Mark (Negative marking: -0.33 marks).

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