Section 4: Bioprocess Engineering and Process Biotechnology
Mass transfer in bioprocesses (kLa)
Subtopic: Fundamentals of Mass transfer in bioprocesses (kLa)
Question Statement:
Determine the correctness or otherwise of the following Assertion (a) and the Reason (r)
Assertion:
formation by DNA fragmentation.
MTT assay is used to determine cell viability based on the principle of colour
Reason:
converting tetrazolium soluble salt 10 insoluble salt
MTT assay is used to determine cell viability based on ite colour development b
Options:
(A) both (a) and (r) are true and (r) is the
(B) both (a) and (r) are true and (r) is not the
correct reason for (a)
(C) (a) is true but (r) is false
(D) (a) is false but (r) is true
correct reason for (a)
Official Answer Key:
A
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2010 Q.29)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: Biotechnology
- **Question Type**: **MCQ** (2 Marks)
- **Core Topic**: Biochemistry, Microbiology & Molecular Biology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"Determine the correctness or otherwise of the following Assertion (a) and the Reason (r)
Assertion:
formation by DNA fragmentation.
MTT assay is used to determine cell viability based on the principle of colour
Reason:
c..."*
- **Option (A) [CORRECT]**: *"both (a) and (r) are true and (r) is the"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (B) [INCORRECT]**: *"both (a) and (r) are true and (r) is not the
correct reason for (a)"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (C) [INCORRECT]**: *"(a) is true but (r) is false"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (D) [INCORRECT]**: *"(a) is false but (r) is true
correct reason for (a)"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **A**
- **Scoring Criteria**: 2 Marks (Negative marking: -0.66 marks).
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