Subtopic: Fundamentals of Biomolecules, enzyme kinetics
Question Statement:
The lurnover numbers for the enzymes. El and E2 are 150 s"' and 15 s" respectively. This means
Options:
(A) El binds to its substrate with higher
(B) The velocity of reactions catalyzed by El and
affinily than E2
concentrations could be equal. if concentration
E2 al their respective saturating substrate
of E2 used is 10 times that of El
(C) The velocity of El catalyzed reaction
(D) The velocity of El catalyzed reaction at i
is always greater than that of E2
pasticular
enzyme concentration
than that of E2 catalyzed reaction under the
saturating substrate concentration is lower
same conditions
Official Answer Key:
B
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2010 Q.35)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: Biotechnology
- **Question Type**: **MCQ** (2 Marks)
- **Core Topic**: Biochemistry, Microbiology & Molecular Biology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"The lurnover numbers for the enzymes. El and E2 are 150 s"' and 15 s" respectively. This means..."*
- **Option (B) [CORRECT]**: *"The velocity of reactions catalyzed by El and
affinily than E2
concentrations could be equal. if concentration
E2 al their respective saturating substrate
of E2 used is 10 times that of El"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (A) [INCORRECT]**: *"El binds to its substrate with higher"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (C) [INCORRECT]**: *"The velocity of El catalyzed reaction"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (D) [INCORRECT]**: *"The velocity of El catalyzed reaction at i
is always greater than that of E"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **B**
- **Scoring Criteria**: 2 Marks (Negative marking: -0.66 marks).
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