GATE BT 2010 • Q52 MCQ • 2 Marks

Section 2: General Biotechnology

Biomolecules, enzyme kinetics

Subtopic: Fundamentals of Biomolecules, enzyme kinetics

Question Statement:

The AG° for the oxidation of NADH by FAD is

Options:

  • (A) -9.25 KJ mol'
  • (B) - 103.04 kJ mol"'
  • (C) + 51.52 kJ mol'
  • (D) - 18.5 kJ mol"

Official Answer Key:

D

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2010 Q.52)** **1. Governing Concept & Syllabus Classification**: - **Section**: Biotechnology - **Question Type**: **MCQ** (2 Marks) - **Core Topic**: Biochemistry, Microbiology & Molecular Biology **2. Detailed Step-by-Step Analytical & Scientific Derivation**: - **Systematic Conceptual & Option-by-Option Evaluation**: - Problem Focus: *"The AG° for the oxidation of NADH by FAD is..."* - **Option (D) [CORRECT]**: *"- 18.5 kJ mol""* - Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required. - **Option (A) [INCORRECT]**: *"-9.25 KJ mol'"* does not satisfy the precise boundary conditions or molecular specificity. - **Option (B) [INCORRECT]**: *"- 103.04 kJ mol"'"* does not satisfy the precise boundary conditions or molecular specificity. - **Option (C) [INCORRECT]**: *"+ 51.52 kJ mol'"* does not satisfy the precise boundary conditions or molecular specificity. **3. Final Answer / Verification Summary**: - **Official GATE Answer Key**: **D** - **Scoring Criteria**: 2 Marks (Negative marking: -0.66 marks).

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