Section 3: Genetics, Cellular and Molecular Biology
Recombinant DNA technology
Subtopic: Fundamentals of Recombinant DNA technology
Question Statement:
Consider the following statements:
[I] All enzymes are proteins.
[II] Ribozymes are catalytic RNA molecules.
Options:
(A) Only [I] is true
(B) Only [II] is true
(C) Both [I] and [II] are true
(D) [I] is false and [II] is true
Official Answer Key:
D
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2010 Q.34)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: Biotechnology
- **Question Type**: **MCQ** (2 Marks)
- **Core Topic**: Biochemistry, Microbiology & Molecular Biology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"Consider the following statements.
II. The ligation efficiency of T4 DNA ligase can be increased with PEG and ficoll
L. TA DNA ligase can catalyze blual end ligation more efficiently than E.coli DNA ligase..."*
- **Option (B) [CORRECT]**: *"both I and Il are true"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (A) [INCORRECT]**: *"only lis true
only ll is true"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (D) [INCORRECT]**: *"l is true and Il is false"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **B**
- **Scoring Criteria**: 2 Marks (Negative marking: -0.66 marks).
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