GATE BT 2010 • Q18 MCQ • 1 Mark

Section 3: Genetics, Cellular and Molecular Biology

Recombinant DNA technology

Subtopic: Fundamentals of Recombinant DNA technology

Question Statement:

Expressed Sequence Tag is defined as

Options:

  • (A) a partial sequence of a cDNA randomly selected from cDNA library
  • (B) an uncharacterized fragment of DNA presence in the cell
  • (C) the characteristic gene expressed in the cell
  • (D) the protein coding DNA sequence of a cDNA

Official Answer Key:

A

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2010 Q.18)** **1. Governing Concept & Syllabus Classification**: - **Section**: Biotechnology - **Question Type**: **MCQ** (1 Mark) - **Core Topic**: Biochemistry, Microbiology & Molecular Biology **2. Detailed Step-by-Step Analytical & Scientific Derivation**: - **Systematic Conceptual & Option-by-Option Evaluation**: - Problem Focus: *"Expressed Sequence Tag is defined as..."* - **Option (A) [CORRECT]**: *"i partial sequence of a codon randomly selected from cDNA"* - Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required. - **Option (B) [INCORRECT]**: *"the characteristic gene expressed in the (0) library uncharacterized fragme"* does not satisfy the precise boundary conditions or molecular specificity. **3. Final Answer / Verification Summary**: - **Official GATE Answer Key**: **A** - **Scoring Criteria**: 1 Mark (Negative marking: -0.33 marks).

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