Section 3: Genetics, Cellular and Molecular Biology
Recombinant DNA technology
Subtopic: Fundamentals of Recombinant DNA technology
Question Statement:
Match Group I with Group II:
Group I:
P. Western blotting
Q. Northern blotting
R. Southern blotting
S. Dot blotting
Group II:
1. RNA detection
2. Protein detection
3. DNA detection
4. Rapid profiling without gel electrophoresis
Options:
(A) P-2, Q-1, R-3, S-4
(B) P-3, Q-2, R-1, S-4
(C) P-1, Q-2, R-4, S-3
(D) P-4, Q-1, R-2, S-3
Official Answer Key:
A
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2010 Q.32)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: Biotechnology
- **Question Type**: **MCQ** (2 Marks)
- **Core Topic**: Biochemistry, Microbiology & Molecular Biology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"Match Group I with Group Il
Group I
Group Il
P. Fibronectin
R. a-Macroglobilin
Q. Insulin
Uptake of amino acids and gluco
Trypsin inhibitc
S. Transferrin
3. Binds iron
4. Cell attachment 10 substralum..."*
- **Option (D) [CORRECT]**: *"P-4. Q-1. R-2, S."*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (C) [INCORRECT]**: *"P.2, Q-1. R-4, S-
P-4. Q-2. R-1, S-"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (B) [INCORRECT]**: *"P-3. Q-2. R-1. 5."* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **D**
- **Scoring Criteria**: 2 Marks (Negative marking: -0.66 marks).
Practice This in Real CBT Exam Interface
Test yourself with the official TCS-iON palette, virtual calculator, and immediate performance analytics.