Section 3: Genetics, Cellular and Molecular Biology
DNA replication, transcription, translation
Subtopic: Fundamentals of DNA replication, transcription, translation
Question Statement:
Match the following antibiotics in Group I with their mode of action in Group II:
Group I:
P. Chloramphenicol
Q. Rifampicin
R. Tetracycline
S. Ciprofloxacin
Group II:
1. Inhibits RNA polymerase
2. Inhibits peptidyl transferase
3. Inhibits DNA gyrase
4. Inhibits aminoacyl-tRNA binding to A-site
Options:
(A) P-2, Q-1, R-4, S-3
(B) P-1, Q-3, R-2, S-4
(C) P-3, Q-1, R-2, S-4
(D) P-4, Q-2, R-3, S-1
Official Answer Key:
A
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2010 Q.30)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: Biotechnology
- **Question Type**: **MCQ** (2 Marks)
- **Core Topic**: Biochemistry, Microbiology & Molecular Biology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"Match the following antibiotics in Group E with their mode of action in Group Il
Group I
Group Il
P. Chloramphenicol
). Norfloxaci
1. Binds to DNA gyrase
Puromycil
2. Binds to RNA Polymerase
S. Rifampicin
4. Mimics aminc..."*
- **Option (C) [CORRECT]**: *"P.3. Q-1, R-2, S-4
P-3, Q-1, R-4, S-2"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (A) [INCORRECT]**: *"P-1. Q-3, R-2, S-4"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (D) [INCORRECT]**: *"P4. Q-2.R-3.5-1"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **C**
- **Scoring Criteria**: 2 Marks (Negative marking: -0.66 marks).
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