Bioprocess Engineering & Kinetics

a chenion growing der steady sale a bacterial culture can a grown a divin a higher

-D Electrophoresis 1. Biochips S. Microarray R. Affinity chromatography 2. Syber Green 3. Antibody linked sephrose beads 4. Ampholyles

Program used for essentially local similarity search is

Peptidyl transferase activity resides in

Under stress conditions bacteria accumulate

Which one of the following DOES NOT belong to the domain of Bacteria?

Interferon-beta (IFN-β) is a crucial type I interferon cytokine primarily produced by:

Accession number is a unique identification assigned to a

In a chemostat growing under steady state, a bacterial culture can be grown at a dilution rate higher than the maximum specific growth rate by:

Idenify the enzyme that catalyzes the following reaction a-Keloglutarate + NADH + NH, + H° ? Glutamate + NAD' + H-O

The degree of inhibition for an enzyme catalyzed reaction at a particular inhibitor concentration is independent of initial substrate concentration. The inhibition follows

Nuciease-hypersensitive sites in the chromosoines are sites that appear to be

The formation of peptide cross-links bervicen adjacent glycan chains in cell wall synthesis is called

Decermine the correctness or otherwise of the following Assertion (a) and the Reason (r) Assertion: Somatic embryogenesis in plants is a two slep process comprising of embryc Reason: Embryo initiation is indepeudent of the presence of 2. 4-dichlorophenoxyacetic acid whereas embryo production requires a high concentration of 2.4. dichlorophenox vacetic acid.

An immobilized enzyme being used in a continuous plug low reactor exhibits an effectiveness factor (m) of 1.2. The value of y being greater than I 0) could be apparently due to

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r) Assertion: formation by DNA fragmentation. MTT assay is used to determine cell viability based on the principle of colour Reason: converting tetrazolium soluble salt 10 insoluble salt MTT assay is used to determine cell viability based on ite colour development b

Match the chemicals in Group I with the possible type/class in Group II Group ! Group Il P. Picloram Q. Zeatin 2. Auxin 1. Vitamin R. Thiamine S. Glutamine 3. Amino Acid 4. Cytokinin

Match Group I with Group Il Group I Group Il P. Fibronectin R. a-Macroglobilin Q. Insulin Uptake of amino acids and gluco Trypsin inhibitc S. Transferrin 3. Binds iron 4. Cell attachment 10 substralum

The lurnover numbers for the enzymes. El and E2 are 150 s"' and 15 s" respectively. This means

Maich the iterns in Group I with Group 11 Group I (Vectors) Group II (Maximum DNA packaging) P. A phage Q. Bacterial Artificial Chromosomes (BACs) 1. 35-45 kb 2. 100-300 kb S. R. Pl derived Artificial Chromosomes (PACS) 3. ≤ 300 kh à cosmid 4. 5-25 kh ()

Match Group I with Group li Group! Group lI P. Staphylococcus aureus R. Mycobacterium tuberculosis Q. Candida albicans 1. Biofilms 3. Methicillin resistance 2. Bacteriocins S. Lacsobacillus lacsis 4. Isoniazid

A mutant Ga protein with increased GPase activity would

Match the products in Group I with the microbial culures in Group II used for cheir industrial production Group! P. Gluconic acid Group Il 1. Leuconostoc resenteroids Q. L - Lysine R. Dextran 2. Aspergillus niger S. Cellulase 3. Brevibacteriam flavum 4. Trichoderma reesei

Thermal death of microorganisms in liquid medium follows first order kinetics. If the initial cell concentration in the fermentation medium is 10^7 cells/ml and the final acceptable contamination level is 10^-3 cells, for how long should 1 m^3 medium be treated at 120 °C (thermal deactivation rate constant = 0.23 / min) to achieve acceptable load?

True breeding Drosophila flies with curved wings and dark bodies were mated with true breeding short wings and tan body Drosophila. The F1 progeny was observed to be with curved wings and tan body. The F1 progeny was again allowed to interbreed and produced flies of following phenotypes. What is the recombination frequency between wing shape and body colour?

The Now rate required lo result in steady state concentration of sucrose as 1.5 g / L in she bioreactor will be

If Y, = 0.4 g / g for the above culture and steady state ceil concentration in the bioreactor is 4 g / L the resulting substrate concentration will be

The number of c-helical turns permeating the membrane is

The AG° for the oxidation of NADH by FAD is

The value of AG, given Kay its 1.7. al 23°C will be

The mean value of acid concentration will be

Embryonic stem cells are derived from

Apoptosis is a form of programmed cell death characterized by specific morphological and biochemical features including:

Yeast artificial chromosomes (YAC's) are used for cloning

'- 3' polymerization R. 3' - 5' exonuclease activity S. 5' - 3' exonuclease activity

1 Potency (Probability that microbe will overcome human immunity system) A pharmaceutical company is contemplating the development of a vaccine against the most dangerous microbe. Which microbe should the company target in its first attempt?

Hydrated synthetic seeds which are produced by ion exchange reaction involve mixing the somatic embryos in a solution of

Shoot organogenesis by tissue culture results into

'Hairy roots' induced in vitro by the infection of Agrobacterium rhizogenes, are characterized by: P. a high degree of lateral branching Q. genetic instability of culture R. an absence of geotropism S. poor biomass production

In balanced growth phase of a cell P. all components of a cell grow at the same rate Q. specific growth determined by cell number or cell mass would be the same R. the growth rate is independent of substrate concentration S. the growth rate decreases with decreasing substrate concentration