GATE BT 2011 • Q4 MCQ • 1 Mark

Section 2: General Biotechnology

Biomolecules, enzyme kinetics

Subtopic: Fundamentals of Biomolecules, enzyme kinetics

Question Statement:

Yeast artificial chromosomes (YAC's) are used for cloning

Options:

  • (A) large segments of DNA
  • (B) mRNA
  • (C) bacterial DNA
  • (D) yeast DNA

Official Answer Key:

A

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2011 Q.4)** **1. Governing Concept & Syllabus Classification**: - **Section**: Biotechnology - **Question Type**: **MCQ** (1 Mark) - **Core Topic**: Quantitative & Analytical Aptitude **2. Detailed Step-by-Step Analytical & Scientific Derivation**: - **Systematic Conceptual & Option-by-Option Evaluation**: - Problem Focus: *"Yeast artificial chromosomes (YAC's) are used for cloning..."* - **Option (A) [CORRECT]**: *"large segments of DNA"* - Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required. - **Option (B) [INCORRECT]**: *"mRNA"* does not satisfy the precise boundary conditions or molecular specificity. - **Option (C) [INCORRECT]**: *"bacterial DNA"* does not satisfy the precise boundary conditions or molecular specificity. - **Option (D) [INCORRECT]**: *"yeast DNA"* does not satisfy the precise boundary conditions or molecular specificity. **3. Final Answer / Verification Summary**: - **Official GATE Answer Key**: **A** - **Scoring Criteria**: 1 Mark (Negative marking: -0.33 marks).

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