GATE BT 2011 Question Paper & Solutions

Biomolecules, enzyme kinetics

Embryonic stem cells are derived from

Cell structure and signaling

Members of the antibody protein family that have common structural features are collectively known as

Downstream processing (chromatography, filtration)

Apoptosis is a form of programmed cell death characterized by specific morphological and biochemical features including:

Biomolecules, enzyme kinetics

Yeast artificial chromosomes (YAC's) are used for cloning

Biomolecules, enzyme kinetics

'- 3' polymerization R. 3' - 5' exonuclease activity S. 5' - 3' exonuclease activity

Bioenergetics and Metabolism

An alternative to glycolysis pathway is

Cell structure and signaling

A cell in G, of interphase has 12 chromosomes. How many chromatids will be found per cell during metaphase Il of meiosis?

Biomolecules, enzyme kinetics

1 Potency (Probability that microbe will overcome human immunity system) A pharmaceutical company is contemplating the development of a vaccine against the most dangerous microbe. Which microbe should the company target in its first attempt?

Biomolecules, enzyme kinetics

Hydrated synthetic seeds which are produced by ion exchange reaction involve mixing the somatic embryos in a solution of

Biomolecules, enzyme kinetics

Shoot organogenesis by tissue culture results into

Mass transfer in bioprocesses (kLa)

'Hairy roots' induced in vitro by the infection of Agrobacterium rhizogenes, are characterized by: P. a high degree of lateral branching Q. genetic instability of culture R. an absence of geotropism S. poor biomass production

Biomolecules, enzyme kinetics

In balanced growth phase of a cell P. all components of a cell grow at the same rate Q. specific growth determined by cell number or cell mass would be the same R. the growth rate is independent of substrate concentration S. the growth rate decreases with decreasing substrate concentration

Biomolecules, enzyme kinetics

In N-linked glycosylation, the oligosaccharide chain is attached to protein by

Biomolecules, enzyme kinetics

Restriction endonucleases which recognize and cut same recognition sequences are known

Microbial growth kinetics

Substrate consumption in lag phase of microbial growth is primarily used for P. turn over of the cell material Q. maintenance of intracellular pH R. motility S. increase in cell number

Bioreactor types, scale-up

Wash out (as defined by D=max) of a continuous stirred tank fermenter is characterized by (X=biomass, S=substrate concentration in bioreactor, So=substrate concentration in feed, P=product concentration in bioreactor)

Biomolecules, enzyme kinetics

The study of evolutionary relationships is known as

Biomolecules, enzyme kinetics

The lipopolysaccharides present in bacterial cell wall has lipid A which is connected to

Biomolecules, enzyme kinetics

Molecular chaperones are class of proteins that facilitate

Biomolecules, enzyme kinetics

Gas vacuoles are specialized proteinaceous intracellular nano-compartments present in aquatic prokaryotes for buoyancy regulation, found in:

Linear Algebra

In ABO blood group system, antigenic determinants are

Biomolecules, enzyme kinetics

The most widely used program for multiple sequence alignment is

DNA replication, transcription, translation

Diphtheria toxin, tetracycline and streptomycin inhibit

Biomolecules, enzyme kinetics

The polymorphic domains for Class II MHC proteins are

Biomolecules, enzyme kinetics

The protein in eukaryotes which is subjected to degradation undergoes

Cell structure and signaling

Match the viruses in Group I with their host cell receptors in Group II. Group I Group I P. Hepatitis A virus 1. Heparan sulphate Q. Human immunodeficiency virus R Rabies virus 2. Acetylcholine receptor 3. CD4 protein S. Herpes simplex virus type I 4. Alpha-2 macroglobulin

Microbial growth kinetics

Match the microbial growth characteristics in Group I with the corresponding features in Group II. Group I Group I P. Growth associated product formation 1. Specific growth rate decreases with increasing product concentration Q. Non growth associated product formation 2. Specific product formation rate is constant R. Product inhibition 3. Specific product formation rate is proportional to specific growth rate S. Substrate inhibition 4. Specific growth rate decreases with increasing substrate concentration

Downstream processing (chromatography, filtration)

Match the items in Group I with Group II. Group I Group Il P. Circular dichroism 1. Concentration Q. X-ray crystallography 2. Sedimentation coefficient R. Freeze-drying 3. Secondary structure determination S. Ultracentrifugation 4. Tertiary structure determinatior

Biomolecules, enzyme kinetics

Match the products in Group I with their respective organisms in Group II. Group I Group II P. Glycerol Q. Glutamic acid 1. Corynebacterium glutamicum R. Curdlan Alcaligenes faecali. 3. unaliella salin S. Amphotericin B 4. Streptomyces nodosus

Biomolecules, enzyme kinetics

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r). Assertion: IaM is found in serum as a pentameric protein consisting of five IgM monomers. Reason : The pentameric form of IgM is due to cross-linking of I‹M monomers via peptide bond.

Biomolecules, enzyme kinetics

Determine the correctness or otherwise of the following Assertion (a) and the Reason (T). Assertion : N-methyl-N'-nitro-N-nitrosoguanidine (NTG) is an effective chemical mutagen. Reason : Mutations induced by NTG mainly are the GC → AT transitions.

DNA replication, transcription, translation

Determine the correctness of the following statements I. Enhancer sequences are those DNA sequences that are involved in increasing the rate of DNA replication. II. Enhancer sequences work by binding with eukaryotic gene activator factors.

Mass transfer in bioprocesses (kLa)

In a well aerated and agitated microbial culture, the 'supply' of oxygen is equal to 'demand' (uptake) of the growing culture. The KLa for such a system will be (KLa = volumetric mass transfer coefficient, C* = dissolved oxygen concentration in liquid in equilibrium with gaseous oxygen, C = instantaneous value of dissolved oxygen concentration, 'I' = specific oxygen uptake rate per unit weight of cells, X = dry weight of the cells per unit volume)

Biomolecules, enzyme kinetics

Structured William's model P. can describe the changes in intracellular components of the cell during growth Q. can not describe the death phase of the cells R. can describe the variation of size of cells in the different phases of growth S. can not describe the lag period of growth Which one of the following is CORRECT?

Biomolecules, enzyme kinetics

Match items in Group I with Group II. Group I Group Il P. Glycolytic pathway 1. Chloroplast Q. Eukaryotic oxidative metabolism 2. Glyoxysomes R. Glyoxylate cycle 3. Mitochondria S. Calvin cycle 4. Cytosol

Biomolecules, enzyme kinetics

Match items in Group I with Group II. Group! Group II P. Alzheimer's disease 1. HINI Q. Mad cow disease .. Hemoglobi R. Sickle cell anaemia 3. Prions S. Swine flu 4. Amyloid

DNA replication, transcription, translation

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r) Assertion: The elucidation of ribosome structure helps in the development of new generation drugs. Reason: The high resolution of macromolecular structure has enabled in structure-based drug design.

Biomolecules, enzyme kinetics

Determine the correctness or otherwise of the following Assertion (a) and Reason (r): Assertion (a): A very low amount of inhibitor can act as an activator for allosteric enzymes. Reason (r): Allosteric enzymes follow Michaelis-Menten kinetics.

DNA replication, transcription, translation

Match the terms in Group I with their associated functions in Group II. Group I Group II Q. Leucine zipper P. Shine-Dalgarno sequences 1. Aminoacylation of tRNA 2. Gene silencing R. Aminoacyl tRNA synthetase 3. Ribosome binding and facilitation of S. RNA interference (RNAi) translation initiation 4. Transcription factors

Recombinant DNA technology

Protein-protein interactions are studied by P. DNA foot printing Q. Yeast two hybrid system R. Ligase chain reaction S. Mass spectrometry

Biomolecules, enzyme kinetics

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r) Reason : Assertion: Isopropylthiogalactoside (IPTG) is a gratuitous inducer of lactose operon. Gratuitous inducers are chemical analogs which behave like natural inducer but they do not serve as substrate for the enzymes that are subsequently synthesized.

Biomolecules, enzyme kinetics

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r) Assertion: In synchronous culture, majority of the cells move to next phase of the cell Reason : cycle simultaneously. Synchronous culture could be obtained by starving cells for essential nutrient components.

Recombinant DNA technology

Which of the following characteristics with respect to bacterial DNA polymerases is/are TRUE? P. DNA Polymerase I has 5' to 3' exonuclease proofreading activity Q. DNA Polymerase III is the primary replicative polymerase R. DNA Polymerase I requires RNA primers for in vivo initiation S. DNA Polymerase II has reverse transcriptase activity

Microbial growth kinetics

Maximum specific growth rate (max) of a microorganism is calculated by taking the (In=l0ge, X=biomass, t = time)

Biomolecules, enzyme kinetics

Identify the CORRECT statements P. 5' and 3' ends of the transcripts can be mapped by utilizing polymerase chain Q. S, nuclease can cleave the DNA strand of a DNA-RNA hybrid R. 14 polynucleotide kinase is used for labeling 3' end of DNA S. Baculovirus (Autographa californica) can be used as an insect expression vector

Linear Algebra

Value of the determinant mentioned below is 202 11

Mass transfer in bioprocesses (kLa)

HAT (hypoxanthine, aminopterin and thymidine) is used for selecting the hybridomas based on the following Only hybridoma will grow since it inherited the HGPRT genes from B-cells and can synthesize DNA from hypoxanthine. II. Myeloma cells will not grow in cultures since de novo synthesis is blocked by aminopterin and due to the lack of HGPRT enzyme.

Probability and Statistics

What will be the probability of having the colour-blind son to a woman with phenotypically has no previous children) normal parents and a colour-blind brother, and married to a normal man? (Assume that she

Probability and Statistics

What will be the probability of having the colour-blind daughter to a phenotypically man? normal woman, who already had one colour-blind son, and is married to a colour-blind

Microbial growth kinetics

What would be the yield Yx/s (Kg biomass/Kg substrate)?

Biomolecules, enzyme kinetics

What would be the sucrose concentration in the input feed for the output to be 45 Kg biomass h''?

Probability and Statistics

Variance (Vx) for this population of fruit flies as calculated from the above data shall be

Probability and Statistics

The value of standard deviation (SD) will be

Biomolecules, enzyme kinetics

How many molecules of amplicons will be generated?

Biomolecules, enzyme kinetics

How many molecules of amplicons will be present in 0.1 ul of reaction?

Verbal Aptitude

Which of the following options is the closest in the meaning to the word below: Inexplicable

Verbal Aptitude

Choose the word from the options given below that is most nearly opposite in meaning to the given word: Amalgamate

Verbal Aptitude

Choose the most appropriate word from the options given below to complete the following sentence. If you are trying to make a strong impression on your audience, you cannot do so by being understated, tentative or

Verbal Aptitude

Choose the most appropriate word(s) from the options given below to complete the following sentence. I contemplated Singapore for my vacation but decided against it.

Quantitative Aptitude

If Log (P) = (1/2)Log (Q) = (1/3) Log (R), then which of the following options is TRUE?

Verbal Aptitude

Few school curricula include a unit on how to deal with bereavement and grief, and yet all students at some point in their lives suffer from losses through death and parting. Based on the above passage which topic would NOT be included in a unit on bereavement?

Quantitative Aptitude

A container originally contains 10 litres of pure spirit. From this container 1 litre of spirit is replaced with 1 litre of water. Subsequently, 1 litre of the mixture is again replaced with 1 litre of water and this process is repeated one more time. How much spirit is now left in the container?

Quantitative Aptitude

A transporter receives the same number of orders each day. Currently, he has some pending orders (backlog) to be shipped. If he uses 7 trucks, then at the end of the 4th day he can clear all the orders. Alternatively, if he uses only 3 trucks, then all the orders are cleared at the end of the 10th day. What is the minimum number of trucks required so that there will be no pending order at the end of the 5th day?

Quantitative Aptitude

The variable cost (V) of manufacturing a product varies according to the equation V= 4q, where q is the quantity produced. The fixed cost (F) of production of same product reduces with q according to the equation F = 100/q. How many units should be produced to minimize the total cost (V+F)?

Quantitative Aptitude

P, Q, R and S are four types of dangerous microbes recently found in a human habitat. The area of each circle with its diameter printed in brackets represents the growth of a single microbe surviving human immunity system within 24 hours of entering the body. The danger to human beings varies proportionately with the toxicity, potency and growth attributed to a microbe shown in the figure below: 1000 P (50 mm) 800 600 Toxicity 400 @ (40 mm) R (30 mm) S (20 mm) 200 half of the body mass in kilograms) (milligrams of microbe required to destroy