Subtopic: Fundamentals of Downstream processing (chromatography, filtration)
Question Statement:
Match the items in Group I with Group II.
Group I
Group Il
P. Circular dichroism
1. Concentration
Q. X-ray crystallography
2. Sedimentation coefficient
R. Freeze-drying
3. Secondary structure determination
S. Ultracentrifugation
4. Tertiary structure determinatior
Options:
(A) P-4, Q-1, R-2, S-3
(B) P-1, Q-4, R-3, S-2
(C) P-2, Q-3, R-4, S-1
(D) P-3, Q-4, R-1, S-2
2011
Official Answer Key:
D
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2011 Q.28)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: Biotechnology
- **Question Type**: **MCQ** (2 Marks)
- **Core Topic**: Biochemistry, Microbiology & Molecular Biology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"Match the items in Group I with Group II.
Group I
Group Il
P. Circular dichroism
1. Concentration
Q. X-ray crystallography
2. Sedimentation coefficient
R. Freeze-drying
3. Secondary structure determination
S. Ultracentri..."*
- **Option (D) [CORRECT]**: *"P-3, Q-4, R-1, S-2
BT
5/16
--- PAGE 6 ---
2011"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (A) [INCORRECT]**: *"P-4, Q-1, R-2, S-3"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (B) [INCORRECT]**: *"P-1, Q-4, R-3, S-2"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (C) [INCORRECT]**: *"P-2, Q-3, R-4, S-1"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **D**
- **Scoring Criteria**: 2 Marks (Negative marking: -0.66 marks).
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