Subtopic: Fundamentals of Biomolecules, enzyme kinetics
Question Statement:
Identify the CORRECT statements
P. 5' and 3' ends of the transcripts can be mapped by utilizing polymerase chain
Q. S, nuclease can cleave the DNA strand of a DNA-RNA hybrid
R. 14 polynucleotide kinase is used for labeling 3' end of DNA
S. Baculovirus (Autographa californica) can be used as an insect expression vector
Options:
(A) P and Q only
(B) R and S only
(C) P and S only
(D) Q and R only
Official Answer Key:
C
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2011 Q.45)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: Biotechnology
- **Question Type**: **MCQ** (2 Marks)
- **Core Topic**: Biochemistry, Microbiology & Molecular Biology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"Identify the CORRECT statements
P. 5' and 3' ends of the transcripts can be mapped by utilizing polymerase chain
Q. S, nuclease can cleave the DNA strand of a DNA-RNA hybrid
R. 14 polynucleotide kinase is used for labeli..."*
- **Option (C) [CORRECT]**: *"P and S only"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (A) [INCORRECT]**: *"P and Q only"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (B) [INCORRECT]**: *"R and S only"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (D) [INCORRECT]**: *"Q and R only"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **C**
- **Scoring Criteria**: 2 Marks (Negative marking: -0.66 marks).
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