Section 4: Bioprocess Engineering and Process Biotechnology
Mass transfer in bioprocesses (kLa)
Subtopic: Fundamentals of Mass transfer in bioprocesses (kLa)
Question Statement:
HAT (hypoxanthine, aminopterin and thymidine) is used for selecting the hybridomas
based on the following
Only hybridoma will grow since it inherited the HGPRT genes from B-cells and
can synthesize DNA from hypoxanthine.
II.
Myeloma cells will not grow in cultures since de novo synthesis is blocked by
aminopterin and due to the lack of HGPRT enzyme.
Options:
(A) only I is true
(B) only Ill is true
(C) both I and Il are true
(D) I is true and Ill is false
2011
Common Data Questions
Common Data for Questions 48 and 49:
Red-green colour blindness is inherited as a recessive X-linked trait.
Official Answer Key:
C
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2011 Q.47)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: Biotechnology
- **Question Type**: **MCQ** (2 Marks)
- **Core Topic**: Biochemistry, Microbiology & Molecular Biology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"HAT (hypoxanthine, aminopterin and thymidine) is used for selecting the hybridomas
based on the following
Only hybridoma will grow since it inherited the HGPRT genes from B-cells and
can synthesize DNA from hypoxanthine...."*
- **Option (C) [CORRECT]**: *"both I and Il are true"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (A) [INCORRECT]**: *"only I is true"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (B) [INCORRECT]**: *"only Ill is true"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (D) [INCORRECT]**: *"I is true and Ill is false
BT
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2011
Common Data Questi"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **C**
- **Scoring Criteria**: 2 Marks (Negative marking: -0.66 marks).
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