General Biotechnology, Biochemistry & Microbiology

a chenion growing der steady sale a bacterial culture can a grown a divin a higher

Program used for essentially local similarity search is

Peptidyl transferase activity resides in

Under stress conditions bacteria accumulate

Which one of the following DOES NOT belong to the domain of Bacteria?

Interferon-beta (IFN-β) is a crucial type I interferon cytokine primarily produced by:

A neonatally thymectomized mouse. immunized with proiein antigen shows

Lymphocyles interact with foreign antigens in

Accession number is a unique identification assigned to a

During lactic acid fermentation, net yield of ATP and NADH per mole of glucose is

The degree of inhibition for an enzyme catalyzed reaction at a particular inhibitor concentration is independent of initial substrate concentration. The inhibition follows

Oxidation reduction reactions with positive standard redox potential (AE°) have

Nuciease-hypersensitive sites in the chromosoines are sites that appear to be

The formation of peptide cross-links bervicen adjacent glycan chains in cell wall synthesis is called

Decermine the correctness or otherwise of the following Assertion (a) and the Reason (r) Assertion: Somatic embryogenesis in plants is a two slep process comprising of embryc Reason: Embryo initiation is indepeudent of the presence of 2. 4-dichlorophenoxyacetic acid whereas embryo production requires a high concentration of 2.4. dichlorophenox vacetic acid.

An immobilized enzyme being used in a continuous plug low reactor exhibits an effectiveness factor (m) of 1.2. The value of y being greater than I 0) could be apparently due to

A roller bottle culture vessel perfectly cylindrical in shape having inner radius (r) = 10 em and length (I) = 20 cm was fitted with a spiral film of length (L) = 30 cm and width (W) = 20 cm. If the film can support 10' anchorage dependent cells per cm', the increase in the surface area after fitting the spiral film and the additional number of cells that can be grown respectively are 1200 cm' and 12 x 10' cells

Match the chemicals in Group I with the possible type/class in Group II Group ! Group Il P. Picloram Q. Zeatin 2. Auxin 1. Vitamin R. Thiamine S. Glutamine 3. Amino Acid 4. Cytokinin

Match Group I with Group Il Group I Group Il P. Fibronectin R. a-Macroglobilin Q. Insulin Uptake of amino acids and gluco Trypsin inhibitc S. Transferrin 3. Binds iron 4. Cell attachment 10 substralum

The lurnover numbers for the enzymes. El and E2 are 150 s"' and 15 s" respectively. This means

Maich the iterns in Group I with Group 11 Group I (Vectors) Group II (Maximum DNA packaging) P. A phage Q. Bacterial Artificial Chromosomes (BACs) 1. 35-45 kb 2. 100-300 kb S. R. Pl derived Artificial Chromosomes (PACS) 3. ≤ 300 kh à cosmid 4. 5-25 kh ()

Match Group I with Group li Group! Group lI P. Staphylococcus aureus R. Mycobacterium tuberculosis Q. Candida albicans 1. Biofilms 3. Methicillin resistance 2. Bacteriocins S. Lacsobacillus lacsis 4. Isoniazid

A mutant Ga protein with increased GPase activity would

Dizygotic twins are connected to a single placenta during iheir embryonic development. These wins

The dissociation constant K, for ligand binding to the receptor is 10-' M. The concentration of ligand required for occupying 10% of receptors is

Receptor R is over expressed in CHO cells and analysed for expression. 6 x 10' cells were incubated with its radioactive ligand (specific activity 100 counts per picomole). If the total counts present in cell pellet was 1000 pm. the average number of receptors R per cell is (assume complete saturation of receptors with ligand and one ligand binds to one receptor)

Match the products in Group I with the microbial culures in Group II used for cheir industrial production Group! P. Gluconic acid Group Il 1. Leuconostoc resenteroids Q. L - Lysine R. Dextran 2. Aspergillus niger S. Cellulase 3. Brevibacteriam flavum 4. Trichoderma reesei

The number of amino acid residues present in ibe protein is

The AG° for the oxidation of NADH by FAD is

The value of AG, given Kay its 1.7. al 23°C will be

The mean value of acid concentration will be

Embryonic stem cells are derived from

Members of the antibody protein family that have common structural features are collectively known as

Yeast artificial chromosomes (YAC's) are used for cloning

'- 3' polymerization R. 3' - 5' exonuclease activity S. 5' - 3' exonuclease activity

An alternative to glycolysis pathway is

A cell in G, of interphase has 12 chromosomes. How many chromatids will be found per cell during metaphase Il of meiosis?

1 Potency (Probability that microbe will overcome human immunity system) A pharmaceutical company is contemplating the development of a vaccine against the most dangerous microbe. Which microbe should the company target in its first attempt?

Hydrated synthetic seeds which are produced by ion exchange reaction involve mixing the somatic embryos in a solution of

Shoot organogenesis by tissue culture results into