GATE BT 2013 Question Paper & Solutions

Biomolecules, enzyme kinetics

Under alkaline conditions, DNA is more stable than RNA because

Biomolecules, enzyme kinetics

Which one of the following modifications is common toboth protein and DNA? (A) SUMOylation (B) Nitrosylation (C)Methylation (D) Ubiquitination

Biomolecules, enzyme kinetics

Protein A, which has strong affinity to F c region of immunoglobulin, is extracted from

Cell structure and signaling

The first humanized monoclonal antibody approved for the treatment of breast canceris (A) Rituximab (B) Cetuximab (C) Bevacizumab (D) Herceptin

Biomolecules, enzyme kinetics

Which one of the following aminoacids in proteins does NOT undergo phosphorylation? (A) Ser (B) Thr (C) Pro (D) Tyr

Cell structure and signaling

In vaccine formulations and immunological assays, the role of an adjuvant is to:

Cell structure and signaling

Endogenous antigens are presented on to the cell surface along with (A) MHC-II (B) MHC-I (C) Fcγreceptor (D) complement receptor

Biomolecules, enzyme kinetics

Human genome sequencing project involved the construction of genomic library in (A)bacterial artificial chromosome (B)pBR322 (C) bacteriophage (D) pcDNA3.1

Biomolecules, enzyme kinetics

The nucleotide analogue used in DNA sequencing by chain termination method is

Recombinant DNA technology

In nature, the horizontal gene transfer across bacteria is mediated by (A) gene cloning followed by transformation (B)conjugation and transformation (C) conjugation only (D) transformation only

DNA replication, transcription, translation

Phylum proteobacteria is subdivided into -, -, -, - and -proteobacteria based on (A) G+C content (B) 23S rRNA sequences (C)tRNA sequences (D)16S rRNA sequences 2013 BIOTECHNOLOGY - BT

Bioenergetics and Metabolism

Which one of the following is an ABC transporter? (A)multidrug resistance protein (B) acetylcholine receptor (C)bacteriorhodopsin (D) ATP synthase

Biomolecules, enzyme kinetics

The catalytic efficiency for an enzyme is definedas (A) kcat (B) ௏೘ೌೣ ௞೎ೌ೟ (C) ௞೎ೌ೟ ௄೘ (D) ௞೎ೌ೟ ௏೘ೌೣ

Mass transfer in bioprocesses (kLa)

Of the two diploid species, species I has 36 chromosomes and species II has 28 chromosomes. How many chromosomes would be found in an allotriploid individual? (A) 42 or 54 (B)46 or 50 (C) 74 or 86 (D) 84 or 108

CRISPR-Cas9 gene editing

The RNA primer synthesized during the replication process in bacteria is removed by (A) DNA gyrase (B)primase (C) DNA polymeraseI (D) DNA polymerase II

Linear Algebra

The suitable substitution matrix to align closely related sequences is (A) PAM 250 or BLOSSUM 80 (B) PAM 40 or BLOSSUM 80 (C) PAM 120 or BLOSSUM 40 (D) PAM 250 or BLOSSUM 40

Biomolecules, enzyme kinetics

If     22 11P ,     22 12Q and     31 03R ,which one of the following statements is TRUE? (A) PQ = PR (B) QR= RP (C) QP = RP (D) PQ = QR

Biomolecules, enzyme kinetics

If ௬), then డ௨ డ௫ ൅ డ௨ డ௬ = (A) ௫ + ௬ (B) ௬ (C) ଵ ௘ೣ ା ௘೤ (D) 1

Biomolecules, enzyme kinetics

Hypophosphatemia is manifested by an X-linked dominant allele.What proportion of the offsprings from a normal male and an affected heterozygous female will manifest the disease? (A) ½ sons and ½ daughters (B) all daughters and no sons (C) all sons and no daughters (D) ¼ daughters and ¼ sons

Biomolecules, enzyme kinetics

One of theeigen values of       1218 410P is (A) 2 (B) 4 (C) 6 (D) 8 Q. 21 - Q. 25 are of numerical answer type.

Microbial growth kinetics

A callus of 5 g dry weight was inoculated on semi-solid medium for growth. The dry weight of the callus was found to increase by 1.5 fold after 10 da ys of inoculation. The growth index of the culture is _________ 2013 BIOTECHNOLOGY - BT

Bioreactor types, scale-up

A chemostat is operated at a dilution rate of 0.6 h -1. At steady state, the biomass concentration in the exit stream was found to be 30 g l -1. The biomass productivity (g l -1h-1) after 3h of steady state operation will be ___________

Bioreactor types, scale-up

A batch bioreactor is to be scaled up from 10 to 10,000 liters. The diameter of the large bioreactor is 10 times that of the small bioreactor. The agita tor speed in the small bioreactor is 450 rpm. Determine the agitator speed (rpm) of the large bi oreactor with same impeller tip speed as that of the small bioreactor. _________

Biomolecules, enzyme kinetics

Calculate the percentage sequence identity for the pairwise alignment given below. _______ H E L L O - Y E L L O W

Microbial growth kinetics

In a batch culture, the specific rate of s ubstrate utilization is 0.25 g (g cell mass) -1 h-1 and specific rate of product formation is 0.215 g (g cell mass) -1 h -1. Calculate the yield of product from the substrate(Yp/s). ___________ Q. 26 to Q. 55 carry two marks each.

Mass transfer in bioprocesses (kLa)

Match the commercial microbial sources in Group I with the products in Group II. Group I Group II P. Corynebacteriumlilium 1. 2,3-Butane di-ol Q. Klebsiellaoxytoca 2. Poly- β-hydroxybutyric acid R. Aspergillusniger 3. Glutamic acid S. Alcaligeneseutrophus 4. Citric acid (A) P-3,Q-1,R-2,S-4 (B) P-3,Q-1,R-4,S-2 (C) P-1,Q-3,R-2,S-4 (D) P-1,Q-3,R-4,S-2

Calculus

Match the entries in the Group I with the elution conditions in Group II. Group I Group II P. Ion-exchange chromatography 1. Isocratic solvent Q. Hydrophobic column chromatography 2. Ampholytes R. Gel filtration chromatography 3. Increasing gradient of salt S. Chromatofocusing 4. Decreasing gradient of polarity (A) P-4,Q-1,R-2,S-3 (B) P-4,Q-3,R-1,S-2 (C) P-3,Q-4,R-1,S-2 (D) P-3,Q-4,R-2,S-1 2013 BIOTECHNOLOGY - BT

Bioreactor types, scale-up

Determine the correctness or otherwise of the following Assertion (a) and Reason (r). Assertion: Immobilization of plant cells can enhance secondary metabolite production during bioreactor cultivation. Reason: Immobilization protects the plant cells from shear forces in the bioreactor.

Biomolecules, enzyme kinetics

Match the cell structures in Group I with the organismsin Group II. Group I Group II P. Endospores 1. Methanobacterium Q. Bipolar flagella 2. Treponema R. Pseudomurine in cell wall 3. Spirillum S. Periplasmic flagella 4. Clostridium (A) P-4, Q-3, R-1, S-2 (B) P-4, Q-3, R-2, S-1 (C) P-3, Q-4, R-1, S-2 (D) P-4, Q-1, R-3, S-2

DNA replication, transcription, translation

Match the antibioticsinGroup Iwith the targets inGroup II. Group I Group II P. Sulfonamide 1. Peptidoglycan synthesis Q. Quinolones 2. Peptide chain elongation R. Erythromycin 3. Folic acid biosynthesis S. Cephalosporin 4. Topoisomerase (A) P-3, Q-4, R-1, S-2 (B) P-2, Q-4, R-3, S-1 (C) P-4, Q-1, R-2, S-3 (D) P-3, Q-4, R-2, S-1

CRISPR-Cas9 gene editing

In nature, Agrobacterium tumefaciens mediated infection of plant cells leads to P. crown gall disease in plants Q. hairy root disease in plants R. transfer of T-DNA into the plant chromosome S. transfer of Ri-plasmid into the plant cell (A) S only (B) P and R only (C) Q and S only (D) Q only

Biomolecules, enzyme kinetics

Match the entries inGroup I with the enzymes inGroup II. Group I Group II P. NAD+ 1. Glutathione peroxidase Q. Selenium 2. Nitrogenase R. Pyridoxal phosphate 3. Lactate dehydrogenase S. Molybdenum 4. Glycogen phosphorylase (A) P-3, Q-2, R-4, S-1 (B) P-4, Q-1, R-3, S-2 (C) P-3, Q-1, R-4, S-2 (D) P-3, Q-4, R-2, S-1 2013 BIOTECHNOLOGY - BT

Biomolecules, enzyme kinetics

Match the herbicides in Group Iwith the target enzymesin Group II. Group I Group II P. Glyphosate 1. Nitrilase Q. Bromoxynil 2. Acetolactatesynthetase R. Sulphonylureas 3. Dehalogenase S. Dalapon 4. 5-Enolpyruvyl shikimate3-phosphate synthase (A) P-4, Q-1, R-2, S-3 (B) P-2, Q-1, R-4, S-3 (C) P-4, Q-3, R-2, S-1 (D) P-3, Q-2, R-4, S-1

Biomolecules, enzyme kinetics

The activity of an enzyme was measured by varyin g the concentration of the substrate (S) in the presence of three different concentrations of inhi bitor (I) 0, 2 and 4 mM.The double reciprocal plot given belowsuggests that the inhibitor (I) exhibits 1/[S] 1/Vo [I] = 0 mM [I] = 2 mM [I] = 4 mM 0 (A) substrate inhibition (B) uncompetitive inhibition (C) mixed inhibition (D) competitive inhibition

Biomolecules, enzyme kinetics

Match the entries in Group Iwith the entries in Group II. Group I Group II P. RNAse P 1. Polyadenylation Q. RNase H 2. Splicing R. snRNAs 3. Ribozymes S. CstF 4. DNA-RNAhybrids (A) P-3, Q-4, R-2, S-1 (B) P-4, Q-3, R-2, S-1 (C) P-3, Q-2, R-1, S-4 (D) P-2, Q-4, R-1, S-3

Biomolecules, enzyme kinetics

Determine the correctness or otherwise of the following Assertion (a) and Reason (r). Assertion:UPGMA method produces ultrametric tree.  Reason:Sequence alignment is converted into evolutionary distances in UPGMA method.

Biomolecules, enzyme kinetics

Match the entries in the Group Iwith the entries inGroup II. Group I Group II P. Threading 1. Gene duplication Q. FASTA 2. Fold prediction R. Profile 3. HMM S. Paralogs 4. k-tuple (A) P-2, Q-1, R-3, S-4 (B) P-2, Q-4, R-3, S-1 (C) P-3, Q-4, R-2, S-1 (D) P-1, Q-4, R-3, S-2

Biomolecules, enzyme kinetics

Evaluate xx x 1tanlim  (A)  (B) 1 (C) 0 (D)-1

Differential Equations

The Laplace transform of ݂൅6 is (A) ଵ ௦ ൅ ଶ ௦మ (B) ଷ ௦ െ ଺ ௦మ (C) ଺ ௦ ൅ ଶ ௦మ (D) െ ଺ ௦ ൅ ଶ ௦మ

Biomolecules, enzyme kinetics

The solution of the following set of equations is ൌ 20 ൌ 13 ൌ0 (A) ൌ8 (B) ൌ8 (C) ൌെ 8 (D) ൌെ 3

Biomolecules, enzyme kinetics

The solution to ௗ௬ ௗ௫ݔܿ݁ݏ݋ܿ is (A) y = (c+x) cot x (B) y = (c+x) cosec x (C) y = (c + x) cosec x cot x (D) y = (c+x) ௖௢௦௘௖ ௫ ୡ୭୲ ௫

CRISPR-Cas9 gene editing

A complete restriction digestion of a circular plasmid (5000bp) was carried out with HindIII,BamHIandEcoRIindividually. Restriction digestion yielded following fragments. Plasmid + HindIII→ 1200bp and3800bp Plasmid + BamHI→5000bp Plasmid + EcoRI→ 2500bp The number of sites for EcoRI,BamHIandHindIIIpresent on this plasmid are (A)EcoRI-2, BamHI-1, HindIII-2 (B) EcoRI-1, BamHI-1, HindIII-2 (C)EcoRI-3, BamHI-2, HindIII-1 (D) EcoRI-2, BamHI-2, HindIII-1 2013 BIOTECHNOLOGY - BT Q. 43 - Q. 47 are of numerical answer type.

Biomolecules, enzyme kinetics

The total number of fragments generated by the complete and sequential cleavage of the polypeptide given below by Trypsin followed by CNBr is _________ Phe-Trp-Met-Gly-Ala-Lys-Leu-Pro-Met-Asp-Gly-Arg-Cys-Ala-Gln

Classical Genetics and Epistasis

In a genetic study, 80 people were found to have alleles for polydactyly. Only 36 of them were polydactylous. What is the extent of penetrance percentage? ________

Probability and Statistics

One percent of the cars manufactured by a compa ny are defective. What is the probability (upto four decimals) that more than two cars are defective, if 100 cars are produced? __________

Bioreactor types, scale-up

The maximum cell concentration (g l -1) expected in a bioreactor with initial cell concentration of 1.75 g l-1 and an initial glucose concentration of 125 g l-1 is (Yx/s = 0.6 g cell/g substrate) ________

Biomolecules, enzyme kinetics

A fed batch culture was operated with intermittent addition of glucose solution at a flow rate of 200 ml h-1. The values of K s, m and D are 0.3 g l -1, 0.4 h-1 and 0.1 h-1, respectively. Determine the concentration of growth limiting substrate (gl-1) in the reactor at quasi-steady state. ____________ Common Data Questions Common Data for Questions 48 and 49: A solution was prepared by dissolving 100 mg of protein X in 100 ml of water. Molecular weight of protein X is 15,000 Da; Avogadro's number = 6.022x 10 23.

Biomolecules, enzyme kinetics

Calculate the molarity(M) of the resulting solution. (A)66.6 (B)6.6 (C)0.67 (D)0.067

Cell structure and signaling

The number of moleculespresent in this solution is (A) 40.15x10 19 (B) 6.023 x1019 (C)4.015 x1019 (D) 0.08 x1019 2013 BIOTECHNOLOGY - BT Common Data for Questions 50 and 51: The binding efficiency of three different receptorsR1 , R2 and R3 were tested against a ligand using equilibrium dialysis, with a constant concentration of receptor and varying concen trations of ligand. The Scatchard plot of receptor titration with different concentration of ligand is given below (ris moles of bound ligand per moles of receptor and c is concentration of free ligand) 12 4 3 2 1 r/c R1 R2 R3 r R4 30

Cell structure and signaling

The number of ligand binding sites present on receptors R1 and R3, respectively are (A)1 and 4 (B)1 and 1 (C)4 and 1 (D)2 and 2

Cell structure and signaling

Which one of the receptors has thehighestaffinity for the ligand? (A) R1 (B) R2 (C) R3 (D) R4 Linked Answer Questions Statement for Linked Answer Questions 52 and 53: A DNA fragment of 5000bp needs to be isolated from E.coli (genome size 4x103kb) genomic library.

CRISPR-Cas9 gene editing

The minimum number of independent recombinant clones required to represent this fragment in genomic library are (A) 16x102 (B)12 x102 (C) 8 x102 (D) 1.25 x102

Probability and Statistics

The number of clones to represent this fragment in genomic library with a probability of 95% are (A) 5.9 x103 (B) 4.5 x103 (C) 3.6 x103 (D) 2.4 x103 Statement for Linked Answer Questions 54 and 55: During sterilization of a fermentation medium in a given bioreactorheating= 12.56, cooling = 7.48 and the total value of  required for whole sterilization process is 52, where  is the design criteria.

Biomolecules, enzyme kinetics

What is the value of holding? (A)31.96 (B) 42.32 (C) 52.43 (D) 61.18

Biomolecules, enzyme kinetics

What is the holding period (min) at a k value of 3.36min-1? (A) 10.6 (B) 9.5 (C) 8.4 (D) 7.2 2013 BIOTECHNOLOGY - BT

Quantitative Aptitude

If ൑ 5 and ൑1 1 then which of the following options is TRUE?

Verbal Aptitude

The Headmaster ___________ to speak to you. Which of the following options is incorrect to complete the above sentence?

Quantitative Aptitude

Mahatama Gandhi was known for his humility as

Verbal Aptitude

All engineering students should learn mechanics, mathematics and how to do computation. I II III IV Which of the above underlined parts of the sentence is not appropriate? (A) I (B) II (C) III (D) IV

Quantitative Aptitude

Select the pair that best expresses a relationship similar to that expressed in the pair: water: pipe:: (A) cart: road (B) electricity: wire (C) sea: beach (D) music: instrument 2013 BIOTECHNOLOGY - BT Q. 61 to Q. 65 carry two marks each.

Quantitative Aptitude

Velocity of an object fired directly in upward direction is given by ݐwhere ݐtime) is in seconds. When will the velocity be between 32 m/sec and 64 m/sec? (A) (1, 3/2) (B) (1/2, 1) (C) (1/2, 3/2) (D) (1, 3)

Quantitative Aptitude

In a factory, two machines M1 and M2 manufacture 60% and 40% of the autocomponents respectively. Out of the total production, 2% of M1 and 3% of M2 are found to be defective. If a randomly drawn autocomponent from the combined lo t is found defective, what is the probability that it was manufactured by M2? (A) 0.35 (B) 0.45 (C) 0.5 (D) 0.4

Quantitative Aptitude

Following table gives data on tourists from different countries visiting India in the year 2011. Country Number of Tourists USA 2000 England 3500 Germany 1200 Italy 1100 Japan 2400 Australia 2300 France 1000 Which two countries contributed to the one third of the total number of tourists who visited India in 2011?

Quantitative Aptitude

If ൅ 9| ൌ3 then the possible value of ଶ would be: (A) 30 (B) -30 (C) -42 (D) 42

Analytical & Spatial Aptitude

All professors are researchers Some scientists are professors Which of the given conclusions is logically valid and is inferred from the above arguments: