Section 3: Genetics, Cellular and Molecular Biology
CRISPR-Cas9 gene editing
Subtopic: Fundamentals of CRISPR-Cas9 gene editing
Question Statement:
The minimum number of independent recombinant clones required to represent this fragment in
genomic library are
(A) 16x102 (B)12 x102 (C) 8 x102 (D) 1.25 x102
Options:
(A) 16x102
(B) 12 x102
(C) 8 x102
(D) 1.25 x102
Official Answer Key:
C
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2013 Q.52)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: BT
- **Question Type**: **MCQ** (2 Marks)
- **Core Topic**: Recombinant DNA Technology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"The minimum number of independent recombinant clones required to represent this fragment in
genomic library are..."*
- **Option (C) [CORRECT]**: *"8 x102"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (A) [INCORRECT]**: *"16x102"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (B) [INCORRECT]**: *"12 x102"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (D) [INCORRECT]**: *"1.25 x102"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **C**
- **Scoring Criteria**: 2 Marks (Negative marking: -0.66 marks).
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