Subtopic: Fundamentals of Biomolecules, enzyme kinetics
Question Statement:
Human genome sequencing project involved the construction of genomic library in
(A)bacterial artificial chromosome (B)pBR322
(C) bacteriophage (D) pcDNA3.1
Options:
(A) bacterial artificial chromosome
(B) pBR322
(C) bacteriophage
(D) pcDNA3.1
Official Answer Key:
A
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2013 Q.8)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: BT
- **Question Type**: **MCQ** (1 Mark)
- **Core Topic**: Quantitative & Analytical Aptitude
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"Human genome sequencing project involved the construction of genomic library in..."*
- **Option (A) [CORRECT]**: *"bacterial artificial chromosome"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (B) [INCORRECT]**: *"pBR322"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (C) [INCORRECT]**: *"bacteriophage"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (D) [INCORRECT]**: *"pcDNA3.1"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **A**
- **Scoring Criteria**: 1 Mark (Negative marking: -0.33 marks).
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