Section 3: Genetics, Cellular and Molecular Biology
CRISPR-Cas9 gene editing
Subtopic: Fundamentals of CRISPR-Cas9 gene editing
Question Statement:
The RNA primer synthesized during the replication process in bacteria is removed by
(A) DNA gyrase (B)primase
(C) DNA polymeraseI (D) DNA polymerase II
Options:
(A) DNA gyrase
(B) primase
(C) DNA polymeraseI
(D) DNA polymerase II
Official Answer Key:
C
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2013 Q.15)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: BT
- **Question Type**: **MCQ** (1 Mark)
- **Core Topic**: Recombinant DNA Technology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"The RNA primer synthesized during the replication process in bacteria is removed by..."*
- **Option (C) [CORRECT]**: *"DNA polymeraseI"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (A) [INCORRECT]**: *"DNA gyrase"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (B) [INCORRECT]**: *"primase"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (D) [INCORRECT]**: *"DNA polymerase II"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **C**
- **Scoring Criteria**: 1 Mark (Negative marking: -0.33 marks).
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