Match the entries in the Group I with the elution conditions in Group II.
Group I
Group II
P. Ion-exchange chromatography 1. Isocratic solvent
Q. Hydrophobic column chromatography 2. Ampholytes
R. Gel filtration chromatography 3. Increasing gradient of salt
S. Chromatofocusing 4. Decreasing gradient of polarity
(A) P-4,Q-1,R-2,S-3 (B) P-4,Q-3,R-1,S-2
(C) P-3,Q-4,R-1,S-2 (D) P-3,Q-4,R-2,S-1
2013 BIOTECHNOLOGY - BT
Options:
(A) P-4,Q-1,R-2,S-3
(B) P-4,Q-3,R-1,S-2
(C) P-3,Q-4,R-1,S-2
(D) P-3,Q-4,R-2,S-1
2013 BIOTECHNOLOGY - BT
Official Answer Key:
C
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2013 Q.27)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: BT
- **Question Type**: **MCQ** (2 Marks)
- **Core Topic**: Bioprocess Engineering
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"Match the entries in the Group I with the elution conditions in Group II.
Group I
Group II
P. Ion-exchange chromatography 1. Isocratic solvent
Q. Hydrophobic column chromatography 2. Ampholytes
R. Gel filtration..."*
- **Option (C) [CORRECT]**: *"P-3,Q-4,R-1,S-2"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (A) [INCORRECT]**: *"P-4,Q-1,R-2,S-3"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (B) [INCORRECT]**: *"P-4,Q-3,R-1,S-2"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (D) [INCORRECT]**: *"P-3,Q-4,R-2,S-1
2013 BIOTECHNOLOGY - BT
BT 7/13"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **C**
- **Scoring Criteria**: 2 Marks (Negative marking: -0.66 marks).
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