Section 4: Bioprocess Engineering and Process Biotechnology
Mass transfer in bioprocesses (kLa)
Subtopic: Fundamentals of Mass transfer in bioprocesses (kLa)
Question Statement:
Choose the CORRECT combination of True (T) and False (F) statements about microcarriers used
in animal cell culture.
P. Higher cell densities can be achieved using microcarriers
Q. Microcarriers increase the surface area for cell growth
R. Microcarriers are used for both anchorage- and nonanchorage-dependent cells
S. Absence of surface charge on microcarriers enhances attachment of cells
(A) P-T, Q-F, R-T and S-F (B) P-T, Q-T, R-F and S-F
(C) P-F, Q-F, R-T and S-T (D) P-F, Q-T, R-F and S-T
Options:
(A) P-T, Q-F, R-T and S-F
(B) P-T, Q-T, R-F and S-F
(C) P-F, Q-F, R-T and S-T
(D) P-F, Q-T, R-F and S-T
Official Answer Key:
B
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2016 Q.41)**
**1. Governing Concept & Question Classification**:
- **Section**: BT
- **Question Type**: **MCQ** (2 Marks)
- **Topic**: Pharmacognosy & Plant Biotechnology (Plant Secondary Metabolites & Source Species)
**2. Detailed Step-by-Step Analysis**:
- Let us match each secondary metabolite in Column-I with its plant producer species in Column-II:
- **P. Morphine**: A benzylisoquinoline narcotic analgesic alkaloid isolated from the opium poppy, **Papaver somniferum**. $\implies$ **P matches 3**.
- **Q. Pyrethrins**: Natural botanical monoterpenoid insecticides obtained from the flowers of the Asteraceae family (including *Chrysanthemum cinerariifolium* and marigold relatives like **Tagetes erecta**). $\implies$ **Q matches 4**.
- **R. Scopolamine (Hyoscine)**: A tropane anticholinergic alkaloid extracted from Solanaceae species, notably Jimson weed, **Datura stramonium**. $\implies$ **R matches 1**.
- **S. Vincristine (and Vinblastine)**: Bisindole microtubule-disrupting antineoplastic alkaloids isolated from the Madagascar periwinkle, **Catharanthus roseus** (formerly *Vinca rosea*). $\implies$ **S matches 2**.
- Compiling the matches: **P-3, Q-4, R-1, S-2**.
- **Option-by-Option Evaluation**:
- **Option (A) [INCORRECT]**: Assigns morphine to Tagetes.
- **Option (B) [CORRECT]**: P-3 (*P. somniferum*), Q-4 (*T. erecta*), R-1 (*D. stramonium*), S-2 (*C. roseus*).
- **Option (C) [INCORRECT]**: Misassigns morphine to Catharanthus.
- **Option (D) [INCORRECT]**: Mismatches scopolamine and pyrethrins.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **B**
- **Scoring Criteria**: +2.0 Marks (Negative marking: -0.67 marks).
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