GATE BT 2016 • Q31 MCQ • 1 Mark

Section 4: Bioprocess Engineering and Process Biotechnology

Bioreactor types, scale-up

Subtopic: Fundamentals of Bioreactor types, scale-up

Question Statement:

Match the secondary metabolites (Column-I) with the corresponding plant species (Column-II). Column-I Column-II P. Morphine 1. Datura stramonium Q. Pyrethrins 2. Catharanthus roseus R. Scopolamine 3. Papaver somniferum S. Vincristine 4. Tagetes erecta (A) P-4, Q-3, R-1, S-2 (B) P-3, Q-4, R-1, S-2 (C) P-2, Q-3, R-4, S-1 (D) P-4, Q-1, R-2, S-3

Options:

  • (A) P-4, Q-3, R-1, S-2
  • (B) P-3, Q-4, R-1, S-2
  • (C) P-2, Q-3, R-4, S-1
  • (D) P-4, Q-1, R-2, S-3

Official Answer Key:

B

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2016 Q.31)** **1. Governing Concept & Question Classification**: - **Section**: BT - **Question Type**: **MCQ** (1 Mark) - **Topic**: Bioprocess Engineering / Bioreactor Agitation (Impeller Selection for High Viscosity Fluids) **2. Detailed Step-by-Step Analysis**: - The choice of impeller in bioprocess reactors depends strictly on fluid rheology and viscosity: 1. **Propellers and Flat-blade Rushton turbines**: Small diameter ($D_i / T \approx 0.33$), operated at high rotational speeds; generate high shear and local turbulence. Highly effective for low-viscosity fluids (
lt; 10^3\text{ cP}$), but in high-viscosity fluids they produce a localized cavern of motion, leaving the bulk fluid unmixed. 2. **Paddles**: Moderate diameter ($D_i / T \approx 0.5 - 0.8$), operated at low speeds for intermediate viscosities up to $10^4\text{ cP}$. 3. **Helical ribbon and Anchor impellers**: Large diameter close to vessel wall ($D_i / T \approx 0.95 - 0.98$), operated at slow rotational speeds. The continuous spiral ribbon forces viscous material to flow axially along the vessel wall and down the core, ensuring positive bulk top-to-bottom displacement without dead zones. - For extremely high viscosity broths (viscosity
gt; 10^5\text{ cP}$, e.g., fungal mycelia, xanthan gum, or heavy polymer solutions), the **helical ribbon impeller** is the most suitable mixing impeller. - **Option-by-Option Evaluation**: - **Option (A) [INCORRECT]**: Marine propellers are axial impellers for low-viscosity fluids (
lt; 1000\text{ cP}$). - **Option (B) [CORRECT]**: Helical ribbon impellers provide positive displacement and uniform bulk turnover for highly viscous non-Newtonian fluids (
gt; 10^5\text{ cP}$). - **Option (C) [INCORRECT]**: Standard paddles cannot eliminate stagnant zones in highly viscous fluids. - **Option (D) [INCORRECT]**: Flat blade turbines lose mixing efficacy in high-viscosity fluids, producing localized channeling. **3. Final Answer / Verification Summary**: - **Official GATE Answer Key**: **B** - **Scoring Criteria**: +1.0 Mark (Negative marking: -0.33 marks).

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