Assuming random distribution of nucleotides, the average number of fragments generated upon
digestion of a circular DNA of size 4.3 × 105 bp with Alul (5' -AGICT-3' ) is _
_× 103
1.6 to 1.7
Official Answer Key:
1.6 to 1.7
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2015 Q.56)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: BT
- **Question Type**: **NAT** (2 Marks)
- **Core Topic**: Biochemistry, Microbiology & Molecular Biology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Mathematical & Quantitative Formulation**:
- Problem Statement: *"Assuming random distribution of nucleotides, the average number of fragments generated upon
digestion of a circular DNA of size 4.3 × 105 bp with Alul (5' -AGICT-3' ) is _
_× 103
1.6 to 1.7..."*
- Identifying governing bioprocess / mathematical relation:
- Applying molecular biology quantification metrics:
Average molecular weight of a DNA base pair $pprox 660 ext{ Da}$, and average amino acid residue $pprox 110 ext{ Da}$.
$\text{Number of base pairs} = \frac{\text{Molecular Weight (Da)}}{660\text{ Da/bp}}$
- **Calculated Quantitative Value**: **1.6 to 1.7**.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **1.6 to 1.7**
- **Scoring Criteria**: 2 Marks (Negative marking: -0.0 marks).
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