GATE BT 2015 • Q49 MCQ • 2 Marks

Section 2: General Biotechnology

Biomolecules, enzyme kinetics

Subtopic: Fundamentals of Biomolecules, enzyme kinetics

Question Statement:

Choose the CORRECT sequence of steps involved in cytoplast production

Options:

  • (A) Digestion of cell wall - protoplast viability → cybrid formation - osmotic stabilizer
  • (B) Osmotic stabilizer → digestion of cell wall - protoplast viability → cybrid formation
  • (C) Protoplast viability → osmotic stabilizer → digestion of cell wall → cybrid formation
  • (D) Osmotic stabilizer → digestion of cell wall → cybrid formation - protoplast viability

Official Answer Key:

B

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2015 Q.49)** **1. Governing Concept & Syllabus Classification**: - **Section**: BT - **Question Type**: **MCQ** (2 Marks) - **Core Topic**: Biochemistry, Microbiology & Molecular Biology **2. Detailed Step-by-Step Analytical & Scientific Derivation**: - **Systematic Conceptual & Option-by-Option Evaluation**: - Problem Focus: *"Choose the CORRECT sequence of steps involved in cytoplast production..."* - **Option (B) [CORRECT]**: *"Osmotic stabilizer → digestion of cell wall - protoplast viability → cybrid formation"* - Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required. - **Option (A) [INCORRECT]**: *"Digestion of cell wall - protoplast viability → cybrid formation - osmotic "* does not satisfy the precise boundary conditions or molecular specificity. - **Option (C) [INCORRECT]**: *"Protoplast viability → osmotic stabilizer → digestion of cell wall → cybrid"* does not satisfy the precise boundary conditions or molecular specificity. - **Option (D) [INCORRECT]**: *"Osmotic stabilizer → digestion of cell wall → cybrid formation - protoplast"* does not satisfy the precise boundary conditions or molecular specificity. **3. Final Answer / Verification Summary**: - **Official GATE Answer Key**: **B** - **Scoring Criteria**: 2 Marks (Negative marking: -0.66 marks).

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