Subtopic: Fundamentals of Biomolecules, enzyme kinetics
Question Statement:
Match the reagents in Group I with their preferred cleavage sites in Group II
Group I
Group II
P) Cyanogen bromide
1) Carboxyl side of methionine
Q) o-lodosobenzoate
2) Amino side of methionine
R) Hydroxylamine
3) Carboxyl side of tryptophan
S) 2-Nitro-S-thiocyanobenzoate
4) Amino side of cysteine
5) Asparagine-glycine bonds
Options:
(A) P-1, Q-3, R-5, S-4
(B) P-2, Q-3, R-1, S-4
(C) P-1, Q-2, R-5, S-4
(D) P-4, Q-2, R-5, S-3
Official Answer Key:
A
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2015 Q.47)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: BT
- **Question Type**: **MCQ** (2 Marks)
- **Core Topic**: Biochemistry, Microbiology & Molecular Biology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"--- PAGE 15 ---
Match the reagents in Group I with their preferred cleavage sites in Group II
Group I
Group II
P) Cyanogen bromide
1) Carboxyl side of methionine
Q) o-lodosobenzoate
2) Amino side of methionine
R) Hydroxy..."*
- **Option (A) [CORRECT]**: *"P-1, Q-3, R-5, S-4"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (B) [INCORRECT]**: *"P-2, Q-3, R-1, S-4"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (C) [INCORRECT]**: *"P-1, Q-2, R-5, S-4"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (D) [INCORRECT]**: *"P-4, Q-2, R-5, S-3
Options :
1. V A
2
* B
3.
C
4. #D"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **A**
- **Scoring Criteria**: 2 Marks (Negative marking: -0.66 marks).
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