Subtopic: Fundamentals of Cell structure and signaling
Question Statement:
The interaction between an antigen (Ag) and a single-chain antibody (Ab) was studied using
Scatchard analysis. The result is shown below.
Y
(Ab-AgI*
[Ag]
→ X
[Ab-Ag] -
The affinity of interaction and the total concentration of antibody, respectively, can be determined
from
Options:
(A) slope and Y-intercept
(B) Y-intercept and slope
(C) X-intercept and slope
(D) slope and X-intercept
Official Answer Key:
D
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2015 Q.35)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: BT
- **Question Type**: **MCQ** (1 Mark)
- **Core Topic**: Biochemistry, Microbiology & Molecular Biology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"--- PAGE 11 ---
The interaction between an antigen (Ag) and a single-chain antibody (Ab) was studied using
Scatchard analysis. The result is shown below.
Y
(Ab-AgI*
[Ag]
→ X
[Ab-Ag] -
The affinity of interaction and the ..."*
- **Option (D) [CORRECT]**: *"slope and X-intercept
Options :
1.
A
2.1
* B
3. * C
4.0 D"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (A) [INCORRECT]**: *"slope and Y-intercept"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (B) [INCORRECT]**: *"Y-intercept and slope"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (C) [INCORRECT]**: *"X-intercept and slope"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **D**
- **Scoring Criteria**: 1 Mark (Negative marking: -0.33 marks).
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