GATE BT 2015 • Q31 MCQ • 1 Mark

Section 2: General Biotechnology

Biomolecules, enzyme kinetics

Subtopic: Fundamentals of Biomolecules, enzyme kinetics

Question Statement:

In a Lineweaver-Burk double reciprocal plot (1/v vs 1/[S]), uncompetitive inhibition is represented by curves that:

Options:

  • (A) Intersect on the 1/v (Y) axis with unchanged Vmax
  • (B) Are parallel lines with decreased apparent Vmax and decreased apparent Km
  • (C) Intersect on the negative 1/[S] (X) axis with unchanged Km
  • (D) Intersect in the second quadrant above the X-axis

Official Answer Key:

B

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2015 Q.31)** **1. Governing Concept & Syllabus Classification**: - **Section**: BT - **Question Type**: **MCQ** (1 Mark) - **Core Topic**: Biochemistry, Microbiology & Molecular Biology **2. Detailed Step-by-Step Analytical & Scientific Derivation**: - **Systematic Conceptual & Option-by-Option Evaluation**: - Problem Focus: *"Which one of the following graphs represents uncompetitive inhibition?..."* - **Option (A) [CORRECT]**: *""* - Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required. - **Option (B) [INCORRECT]**: *"1 [vo] -E we inbior. With inhibitor ------- Without nhibitor Whoutinhibior"* does not satisfy the precise boundary conditions or molecular specificity. - **Option (D) [INCORRECT]**: *"With inhibtors _With inhibitor Withoutinhibitor Witrout inhibitor Options :"* does not satisfy the precise boundary conditions or molecular specificity. **3. Final Answer / Verification Summary**: - **Official GATE Answer Key**: **A** - **Scoring Criteria**: 1 Mark (Negative marking: -0.33 marks).

Practice This in Real CBT Exam Interface

Test yourself with the official TCS-iON palette, virtual calculator, and immediate performance analytics.

Launch CBT Mock Test