GATE BT 2015 • Q30
MCQ • 1 Mark
Section 2: General Biotechnology
Biomolecules, enzyme kinetics
Subtopic: Fundamentals of Biomolecules, enzyme kinetics
Question Statement:
In DNA sequencing reactions using the chain termination method, the ratio of ddNTPs to dNTPs
should be
Options:
-
(A) 0
-
(B) < 1
-
(C) 1
-
(D) >1
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2015 Q.30)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: BT
- **Question Type**: **MCQ** (1 Mark)
- **Core Topic**: Biochemistry, Microbiology & Molecular Biology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"--- PAGE 9 ---
In DNA sequencing reactions using the chain termination method, the ratio of ddNTPs to dNTPs
should be..."*
- **Option (B) [CORRECT]**: *"< 1"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (A) [INCORRECT]**: *"0"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (C) [INCORRECT]**: *"1"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (D) [INCORRECT]**: *">1
Options :
1.
2. 0 B
3. * C
4.1
*D"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **B**
- **Scoring Criteria**: 1 Mark (Negative marking: -0.33 marks).
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