GATE BT 2015 • Q23
MCQ • 1 Mark
Section 3: Genetics, Cellular and Molecular Biology
DNA replication, transcription, translation
Subtopic: Fundamentals of DNA replication, transcription, translation
Question Statement:
Which one of the following features is NOT required in a prokaryotic expression vector?
Options:
-
(A) oriC
-
(B) Selection marker
-
(C) CMV promoter
-
(D) Ribosome binding site
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2015 Q.23)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: BT
- **Question Type**: **MCQ** (1 Mark)
- **Core Topic**: Biochemistry, Microbiology & Molecular Biology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"Which one of the following features is NOT required in a prokaryotic expression vector?..."*
- **Option (C) [CORRECT]**: *"CMV promoter"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (A) [INCORRECT]**: *"oriC"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (B) [INCORRECT]**: *"Selection marker"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (D) [INCORRECT]**: *"Ribosome binding site
Options :
1. * A
2. * B
3. 0 C
4. * D"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **C**
- **Scoring Criteria**: 1 Mark (Negative marking: -0.33 marks).
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