GATE BT 2014 • Q50 MCQ • 2 Marks

Section 2: General Biotechnology

Biomolecules, enzyme kinetics

Subtopic: Fundamentals of Biomolecules, enzyme kinetics

Question Statement:

The pungency of mustard seeds is primarily due to secondary metabolites such as isothiocyanate and nitrile. The pungency is usually felt only when the seeds are crushed. This is because of

Options:

  • (A) Ratio of unsaturated to saturated fatty acids would increase
  • (B) Ratio of unsaturated to saturated fatty acids would decrease
  • (C) Absolute amount of both fatty acids would increase keeping the ratio same
  • (D) Absolute amount of both fatty acids would remain unchanged

Official Answer Key:

B

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2014 Q.50)** **1. Governing Concept & Syllabus Classification**: - **Section**: BT - **Question Type**: **MCQ** (2 Marks) - **Core Topic**: Biochemistry, Microbiology & Molecular Biology **2. Detailed Step-by-Step Analytical & Scientific Derivation**: - **Systematic Conceptual & Option-by-Option Evaluation**: - Problem Focus: *"The pungency of mustard seeds is primarily due to secondary metabolites such as isothiocyanate and nitrile. The pungency is usually felt only when the seeds are crushed. This is because of..."* - **Option (B) [CORRECT]**: *"the pungent chemicals are stored as inactive conjugates and compartmentalized from the enzymes that convert them into active chemicals"* - Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required. - **Option (A) [INCORRECT]**: *"the coat of the intact seeds blocks the pungent volatiles from being releas"* does not satisfy the precise boundary conditions or molecular specificity. - **Option (C) [INCORRECT]**: *"the pungent chemicals are formed only after the reaction with atmospheric o"* does not satisfy the precise boundary conditions or molecular specificity. - **Option (D) [INCORRECT]**: *"the pungent chemicals are formed only after the reaction with atmospheric c"* does not satisfy the precise boundary conditions or molecular specificity. **3. Final Answer / Verification Summary**: - **Official GATE Answer Key**: **B** - **Scoring Criteria**: 2 Marks (Negative marking: -0.66 marks).

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