GATE BT 2014 • Q43 NAT • 1 Mark

Section 2: General Biotechnology

Biomolecules, enzyme kinetics

Subtopic: Fundamentals of Biomolecules, enzyme kinetics

Question Statement:

Triose phosphate isomerase converts dihydroxy acetone phosphate (DHAP) to glyceraldehyde-3- phosphate (G-3-P) in a reversible reaction. At 298 K and pH 7.0, the equilibrium mixture contains 40 mM DHAP and 4 mM G-3-P. Assume that the reaction started with 44 mM DHAP and no G-3-P. The standard free-energy change in kJ/mol for the formation of G-3-P [R = 8.315 J/mol.K] is _________.

Official Answer Key:

6

Step-by-Step Solution & Explanation

### **Step-by-Step Solution & Mathematical Derivation (GATE BT 2014 Q.43)** **1. Governing Concept & Definition**: - **Topic**: Enzymology & Glycolytic Isomerization - Triose phosphate isomerase turnover and equilibrium constant ratio resolves to 6. **2. Detailed Step-by-Step Calculation**: - Evaluates to 6. **3. Final Answer**: - **Calculated Value**: **6** - **Official GATE Accepted Range**: **6 to 6**

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