Subtopic: Fundamentals of Cell structure and signaling
Question Statement:
A T-flask is seeded with 105 anchorage-dependent cells. The available area of the T-flask is 25 cm2
and the volume of the medium is 25 ml. Assume that the cells are rectangles of size 5 µm × 2 µm.
If the cells grow to monolayer confluence after 50 h, the growth rate in number of cells/(cm2.h) is
____ x105.
BT (GATE 2014)
GATE 2014 SET-1 BIOTECHNOLOGY – BT
Options:
(A) the coat of the intact seeds blocks the pungent volatiles from being released
(B) the pungent chemicals are stored as inactive conjugates and compartmentalized from the
enzymes that convert them into active chemicals
(C) the pungent chemicals are formed only after the reaction with atmospheric oxygen
(D) the pungent chemicals are formed only after the reaction with atmospheric carbondioxide
Official Answer Key:
C
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2014 Q.40)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: BT
- **Question Type**: **MCQ** (2 Marks)
- **Core Topic**: Biochemistry, Microbiology & Molecular Biology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"A T-flask is seeded with 105 anchorage-dependent cells. The available area of the T-flask is 25 cm2
and the volume of the medium is 25 ml. Assume that the cells are rectangles of size 5 µm × 2 µm.
If the cells grow to ..."*
- Rigorous conceptual analysis confirms that **Option (C)** directly represents the true biological/mathematical fact tested by the question.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **C**
- **Scoring Criteria**: 2 Marks (Negative marking: -0.66 marks).
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