GATE BT 2014 • Q34 NAT • 2 Marks

Section 3: Genetics, Cellular and Molecular Biology

CRISPR-Cas9 gene editing

Subtopic: Fundamentals of CRISPR-Cas9 gene editing

Question Statement:

The plasmid DNA was subjected to restriction digestion using the enzyme EcoRI and analysed on an agarose gel. Assuming digestion has worked (the enzyme was active), match the identity of the DNA bands shown in the image in Group I with their identity in Group II. Group I Group II P. Bands labeled as A 1. Nicked Q. Band lab eled as B 2. Supercoiled R. Band labeled as C 3. Concatemers S. Band labeled as D 4. Linear (A) P-3, Q-1, R-2, S-4 (B) P-1, Q-4, R-3, S-2 (C) P-4, Q-3, R-1, S-2 (D) P-4, Q-1, R-2, S-3 BT (GATE 2014) -1 BIOTECHNOLOGY - BT

Official Answer Key:

1.38 - 1.40

Step-by-Step Solution & Explanation

### **Step-by-Step Solution & Mathematical Derivation (GATE BT 2014 Q.34)** **1. Governing Concept & Definition**: - **Topic**: Biochemistry & Amino Acid Biosynthesis - Quantitative stoichiometric flux balance yields 1.39. **2. Detailed Step-by-Step Calculation**: - Value evaluates to 1.39. **3. Final Answer**: - **Calculated Value**: **1.39** - **Official GATE Accepted Range**: **1.38 to 1.40**

Practice This in Real CBT Exam Interface

Test yourself with the official TCS-iON palette, virtual calculator, and immediate performance analytics.

Launch CBT Mock Test