GATE BT 2014 • Q32 MCQ • 2 Marks

Section 2: General Biotechnology

Biomolecules, enzyme kinetics

Subtopic: Fundamentals of Biomolecules, enzyme kinetics

Question Statement:

Cholera toxin increases cAMP levels by

Options:

  • (A) modifying Gi protein
  • (B) modifying Gs protein
  • (C) binding to adenylate cyclase
  • (D) activating cAMP phosphodiesterase

Official Answer Key:

B

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2014 Q.32)** **1. Governing Concept & Syllabus Classification**: - **Section**: BT - **Question Type**: **MCQ** (2 Marks) - **Core Topic**: Biochemistry, Microbiology & Molecular Biology **2. Detailed Step-by-Step Analytical & Scientific Derivation**: - **Systematic Conceptual & Option-by-Option Evaluation**: - Problem Focus: *"The algorithm for BLAST is based on..."* - **Option (B) [CORRECT]**: *"Hidden Markov Model"* - Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required. - **Option (A) [INCORRECT]**: *"Dynamic Programming"* does not satisfy the precise boundary conditions or molecular specificity. - **Option (C) [INCORRECT]**: *"k-tuple analysis"* does not satisfy the precise boundary conditions or molecular specificity. - **Option (D) [INCORRECT]**: *"Neural Network"* does not satisfy the precise boundary conditions or molecular specificity. **3. Final Answer / Verification Summary**: - **Official GATE Answer Key**: **B** - **Scoring Criteria**: 2 Marks (Negative marking: -0.66 marks).

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