Section 4: Bioprocess Engineering and Process Biotechnology
Bioreactor types, scale-up
Subtopic: Fundamentals of Bioreactor types, scale-up
Question Statement:
Consider a continuous culture provided with a sterile feed containing 10 mM glucose. The steady
state cell density and substrate concentration at three different dilution rates are given in the table
below.
Dilution rate (h-1) Cell density (g L-1) Substrate concentration (mM)
Q.05 0.248 0.067
Q.5 0.208 1.667
5 0 10
The maximum specific growth rate µm (in h-1), will be _____.
Options:
(A) immunoprecipitation assay
(B) immunodiffusion assay
(C) immunoaggregation assay
(D) immunofixation assay
Official Answer Key:
C
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2014 Q.31)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: BT
- **Question Type**: **MCQ** (1 Mark)
- **Core Topic**: Bioprocess Engineering
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"Reactions between antibodies and antigens that are detected by precipitate formation in an agar gel
are referred as..."*
- **Option (C) [CORRECT]**: *"immunoaggregation assay"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (A) [INCORRECT]**: *"immunoprecipitation assay"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (B) [INCORRECT]**: *"immunodiffusion assay"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (D) [INCORRECT]**: *"immunofixation assay"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **C**
- **Scoring Criteria**: 1 Mark (Negative marking: -0.33 marks).
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