Section 4: Bioprocess Engineering and Process Biotechnology
Bioreactor types, scale-up
Subtopic: Fundamentals of Bioreactor types, scale-up
Question Statement:
A T-flask is seeded with 105 anchorage-dependent cells. The available area of the T-flask is 25 cm2
and the volume of the medium is 25 ml. Assume that the cells are rectangles of size 5 µm × 2 µm.
If the cells grow to monolayer confluence after 50 h, the growth rate in number of cells/(cm2.h) is
____ x105.
BT (GATE 2014)
-1 BIOTECHNOLOGY - BT
Options:
(A) systemic acquired resistance
(B) hypersensitive response
(C) innate immunity
(D) antibody mediated response
Official Answer Key:
A
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2014 Q.30)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: BT
- **Question Type**: **MCQ** (2 Marks)
- **Core Topic**: Bioprocess Engineering
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"Prior exposure of plants to pathogens is known to increase resistance to future pathogen attacks.
This phenomenon is known as..."*
- **Option (A) [CORRECT]**: *"systemic acquired resistance"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (B) [INCORRECT]**: *"hypersensitive response"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (C) [INCORRECT]**: *"innate immunity"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (D) [INCORRECT]**: *"antibody mediated response"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **A**
- **Scoring Criteria**: 2 Marks (Negative marking: -0.66 marks).
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