GATE BT 2014 • Q25 MCQ • 1 Mark

Section 2: General Biotechnology

Biomolecules, enzyme kinetics

Subtopic: Fundamentals of Biomolecules, enzyme kinetics

Question Statement:

The growth medium for mammalian cells contains serum. One of the major functions of serum is to stimulate cell growth and attachment. However, it must be filter sterilized to

Options:

  • (A) remove large proteins
  • (B) remove collagen only
  • (C) remove mycoplasma and microorganisms
  • (D) remove foaming agents BT (GATE 2014) -1 BIOTECHNOLOGY - BT

Official Answer Key:

B

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2014 Q.25)** **1. Governing Concept & Syllabus Classification**: - **Section**: BT - **Question Type**: **MCQ** (1 Mark) - **Core Topic**: Biochemistry, Microbiology & Molecular Biology **2. Detailed Step-by-Step Analytical & Scientific Derivation**: - **Systematic Conceptual & Option-by-Option Evaluation**: - Problem Focus: *"Which of the following essential element(s) is/are required as major supplement to enhance the bioremediation of oil spills by the resident bacteria?..."* - **Option (B) [CORRECT]**: *"Nitrogen and phosphorus"* - Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required. - **Option (A) [INCORRECT]**: *"Sulfur"* does not satisfy the precise boundary conditions or molecular specificity. - **Option (C) [INCORRECT]**: *"Iron"* does not satisfy the precise boundary conditions or molecular specificity. - **Option (D) [INCORRECT]**: *"Carbon"* does not satisfy the precise boundary conditions or molecular specificity. **3. Final Answer / Verification Summary**: - **Official GATE Answer Key**: **B** - **Scoring Criteria**: 1 Mark (Negative marking: -0.33 marks).

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