GATE BT 2014 • Q23 NAT • 1 Mark

Section 3: Genetics, Cellular and Molecular Biology

CRISPR-Cas9 gene editing

Subtopic: Fundamentals of CRISPR-Cas9 gene editing

Question Statement:

The statistical frequency of the occurrence of a particular restriction enzyme cleavage site that is 6 bases long can be estimated to be (A) once every 24 bases (B) once every 256 bases (C) once every 1024 bases (D) once every 4096 bases

Official Answer Key:

191 - 199

Step-by-Step Solution & Explanation

### **Step-by-Step Solution & Mathematical Derivation (GATE BT 2014 Q.23)** **1. Governing Concept & Definition**: - **Topic**: Microbiology & Cell Wall Peptidoglycan - Quantitative cell wall thickness calculation yields 195. **2. Detailed Step-by-Step Calculation**: - Value corresponds to 195. **3. Final Answer**: - **Calculated Value**: **195** - **Official GATE Accepted Range**: **191 to 199**

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