Section 4: Bioprocess Engineering and Process Biotechnology
Bioreactor types, scale-up
Subtopic: Fundamentals of Bioreactor types, scale-up
Question Statement:
Reactions between antibodies and antigens that are detected by precipitate formation in an agar gel
are referred as
(A) immunoprecipitation assay (B) immunodiffusion assay
(C) immunoaggregation assay (D) immunofixation assay
Options:
(A) immunoprecipitation assay
(B) immunodiffusion assay
(C) immunoaggregation assay
(D) immunofixation assay
Official Answer Key:
C
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2014 Q.21)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: BT
- **Question Type**: **MCQ** (1 Mark)
- **Core Topic**: Biochemistry, Microbiology & Molecular Biology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"Since mammalian cells are sensitive to shear, scale-up of a mammalian cell process must consider,
among other parameters, the following (given N = rotations/time, D=diameter of impeller)..."*
- **Option (C) [CORRECT]**: *"𝜋𝜋𝜋𝜋𝐷𝐷2"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (A) [INCORRECT]**: *"𝜋𝜋𝜋𝜋𝜋𝜋"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (B) [INCORRECT]**: *"𝜋𝜋𝑁𝑁2𝐷𝐷"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (D) [INCORRECT]**: *"none of these"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **C**
- **Scoring Criteria**: 1 Mark (Negative marking: -0.33 marks).
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