GATE BT 2014 • Q13 MCQ • 2 Marks

Section 4: Bioprocess Engineering and Process Biotechnology

Downstream processing (chromatography, filtration)

Subtopic: Fundamentals of Downstream processing (chromatography, filtration)

Question Statement:

The solution for the following set of equations is, 5𝑥𝑥+ 4𝑦𝑦+ 10𝑧𝑧= 13 𝑥𝑥+ 3𝑦𝑦+ 𝑧𝑧= 7 4𝑥𝑥−2𝑦𝑦+ 𝑧𝑧= 0

Options:

  • (A) thick peptidoglycan layer prevents insertion of membrane attack complex into the inner membrane
  • (B) Gram-positive bacteria import the membrane attack complex and inactivate it
  • (C) membrane attack complex is degraded by the proteases produced by the Gram-positive bacteria
  • (D) Gram-positive bacteria cannot activate the complement pathway

Official Answer Key:

C

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2014 Q.13)** **1. Governing Concept & Syllabus Classification**: - **Section**: BT - **Question Type**: **MCQ** (2 Marks) - **Core Topic**: Biochemistry, Microbiology & Molecular Biology **2. Detailed Step-by-Step Analytical & Scientific Derivation**: - **Systematic Conceptual & Option-by-Option Evaluation**: - Problem Focus: *"The solution for the following set of equations is, 5𝑥𝑥+ 4𝑦𝑦+ 10𝑧𝑧= 13 𝑥𝑥+ 3𝑦𝑦+ 𝑧𝑧= 7 4𝑥𝑥−2𝑦𝑦+ 𝑧𝑧= 0..."* - **Option (C) [CORRECT]**: *"x=1, y=0, z=2"* - Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required. - **Option (A) [INCORRECT]**: *"x=2, y=1, z=1"* does not satisfy the precise boundary conditions or molecular specificity. - **Option (B) [INCORRECT]**: *"x=1, y=2, z=0"* does not satisfy the precise boundary conditions or molecular specificity. - **Option (D) [INCORRECT]**: *"x=0, y=1, z=2"* does not satisfy the precise boundary conditions or molecular specificity. **3. Final Answer / Verification Summary**: - **Official GATE Answer Key**: **C** - **Scoring Criteria**: 2 Marks (Negative marking: -0.66 marks).

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