GATE BT 2025 • Q64 NAT • 2 Marks

Section 2: General Biotechnology

Biomolecules, enzyme kinetics

Subtopic: Fundamentals of Biomolecules, enzyme kinetics

Question Statement:

An NMR spectrometer operating at proton resonance frequency (𝜈) of 1 GHz will have a magnetic field strength of _________ Tesla (T). The gyromagnetic ratio for proton, γ = 2.675 × 108 T-1 s-1 (Round off to one decimal place)

Official Answer Key:

23.2 to 23.8

Step-by-Step Solution & Explanation

### **Step-by-Step Solution & Mathematical Derivation (GATE BT 2025 Q.64)** **1. Governing Concept & Definition**: - **Topic**: Biophysical Methods & Nuclear Magnetic Resonance (NMR) - The Larmor resonance frequency $\nu$ (in Hz) of a nucleus in an external magnetic field $B_0$ is: $\nu = \frac{\gamma B_0}{2\pi} \implies B_0 = \frac{2\pi \nu}{\gamma}$ where $\gamma$ is the gyromagnetic ratio of the proton. **2. Detailed Step-by-Step Calculation**: - **Given**: - Operating frequency $\nu = 1\text{ GHz} = 10^9\text{ Hz}$ - Gyromagnetic ratio of proton $\gamma = 2.675 \times 10^8\text{ T}^{-1}\text{s}^{-1}$ - Value of $\pi \approx 3.14159$ - **Calculation**: $B_0 = \frac{2 \times \pi \times 10^9\text{ s}^{-1}}{2.675 \times 10^8\text{ T}^{-1}\text{s}^{-1}} = \frac{2 \times 3.14159 \times 10}{2.675} = \frac{62.8318}{2.675} \approx 23.488\text{ Tesla}$ **3. Final Answer**: - **Calculated Value**: **23.5 T** - **Official GATE Accepted Range**: **23.2 to 23.8**

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