GATE BT 2025 • Q14 MCQ • 1 Mark

Section 2: General Biotechnology

Cell structure and signaling

Subtopic: Fundamentals of Cell structure and signaling

Question Statement:

The minimum value of the function f(x) = x + 4 x for x > 0 is

Options:

  • (A) 1
  • (B) 2
  • (C) 3
  • (D) 4

Official Answer Key:

D

Step-by-Step Solution & Explanation

### **Official Solution & Concept Analysis (GATE BT 2025 Q.14)** **1. Governing Concept**: - **Topic**: Microbiology & Bacterial Endospore Resistance - Bacterial endospores (formed by *Bacillus* and *Clostridium*) exhibit extreme resistance to heat, radiation, desiccation, and chemical disinfectants due to: 1. **Dehydrated core**: Water content is only 10–25%. 2. **Calcium dipicolinate (dipicolinic acid complexed with $\text{Ca}^{2+}$)**: Constitutes ~10% of spore dry weight, intercalating into DNA and stabilizing proteins against thermal denaturation. 3. **Small Acid-Soluble Spore Proteins (SASPs)**: Bind and saturate spore DNA, converting it from B-form to A-form, protecting it from UV radiation and heat. 4. **Multi-layered protective proteinaceous coat and thick cortex**. **2. Option Analysis**: - **Option (A) is INCORRECT**: Spores have extremely low water content (dehydrated), not high. - **Option (B) is CORRECT**: High concentration of calcium dipicolinate and SASPs in the core confers extraordinary thermal resistance. - **Option (C) is INCORRECT**: Spores are metabolically dormant, not hyperactive. - **Option (D) is INCORRECT**: Spore coat is composed of dense cross-linked proteins, not thin lipids. **3. Final Answer**: - **Correct Choice**: **B**

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