Match the item (Column I) with its corresponding use (Column II).
Column I Column II
P. Glutamine 1. Detachment of adherent cells
Q. Trypsin 2. Selection of transfected mammalian cell lines
R. Hypoxanthine 3. Source of carbon and nitrogen in animal cell
culture media
S. Neomycin 4. A component of medium for selection of
hybridoma in monoclonal antibody production
Options:
(A) P-3; Q-1; R-4; S-2
(B) P-1; Q-2; R-4; S-3
(C) P-3; Q-1; R-2; S-4
(D) P-2; Q-3; R-1; S-4
Official Answer Key:
A
Step-by-Step Solution & Explanation
### **Official Solution & Concept Analysis (GATE BT 2024 Q.39)**
**1. Governing Concept**:
- **Topic**: Animal Cell Culture & Cell Biology Reagents
- Cell culture reagents and functional roles:
- **P. Glutamine** $\rightarrow$ Essential amino acid functioning as a primary energy substrate and nitrogen donor in culture media $\rightarrow$ **3**
- **Q. Trypsin** $\rightarrow$ Serine protease used for enzymatic cleavage of extracellular matrix adhesion proteins for **detachment of adherent cells** during subculturing $\rightarrow$ **1**
- **R. Dimethyl sulfoxide (DMSO)** $\rightarrow$ Penetrating **cryoprotectant** preventing intracellular ice crystal formation during cell cryopreservation $\rightarrow$ **4**
- **S. Phenol red** $\rightarrow$ **pH indicator** monitoring culture medium acidification (red/pink at pH 7.4, yellow below 6.8, purple above 8.2) $\rightarrow$ **2**
**2. Option Analysis**:
- **Option (A) [P-3; Q-1; R-4; S-2] is CORRECT**: Comprehensive, flawless matching of cell culture components.
- **Option (B) is INCORRECT**: Misassigns glutamine to detachment.
- **Option (C) is INCORRECT**: Inverts DMSO and phenol red.
- **Option (D) is INCORRECT**: Misidentifies trypsin as pH indicator.
**3. Final Answer**:
- **Correct Choice**: **A**
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