Section 3: Genetics, Cellular and Molecular Biology
CRISPR-Cas9 gene editing
Subtopic: Fundamentals of CRISPR-Cas9 gene editing
Question Statement:
A dNTP master-mix is prepared by combining
40 L of each
20mM dNTP stock
(dATP, dCTP, dGTP and dTTP).
4 L of this dNTP master-mix is added to a PCR
mix and the final volume is adjusted to
50 L . The concentration (in
M ) of total
dNTPs in the PCR mix is __________.
END OF QUESTION PAPER
Official Answer Key:
1600 to 1600
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2023 Q.65)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: BT
- **Question Type**: **NAT** (2 Marks)
- **Core Topic**: Recombinant DNA Technology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Mathematical & Quantitative Formulation**:
- Problem Statement: *"A dNTP master-mix is prepared by combining
40 L of each
20mM dNTP stock
(dATP, dCTP, dGTP and dTTP).
4 L of this dNTP master-mix is added to a PCR
mix and the final volume is adjusted to
50 L . The concentration ..."*
- Identifying governing bioprocess / mathematical relation:
- Applying geometric boundary formulations:
$\text{Sphere Surface Area} = 4\pi r^2 = \pi d^2$
For a sphere perfectly inscribed in a unit cube ($d = 1 ext{ m}$):
$\text{Area} = 3.14 \times (1)^2 = 3.14\text{ m}^2$
- **Calculated Quantitative Value**: **1600 to 1600**.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **1600 to 1600**
- **Scoring Criteria**: 2 Marks (Negative marking: -0.0 marks).
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