Section 4: Bioprocess Engineering and Process Biotechnology
Bioreactor types, scale-up
Subtopic: Fundamentals of Bioreactor types, scale-up
Question Statement:
E. coli is cultivated in a chemostat operated at a dilution rate of
10.2 h- . The
values of biomass yield due to oxygen consumption and the steady state biomass
concentration are
10.2 .gg - and
110 .gL - , respectively. The oxygen transfer rate
(in
11..g L h-- ) is ___________.
Official Answer Key:
10 to 10
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2023 Q.48)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: BT
- **Question Type**: **NAT** (2 Marks)
- **Core Topic**: Bioprocess Engineering
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Mathematical & Quantitative Formulation**:
- Problem Statement: *"E. coli is cultivated in a chemostat operated at a dilution rate of
10.2 h- . The
values of biomass yield due to oxygen consumption and the steady state biomass
concentration are
10.2 .gg - and
110 .gL - , respectiv..."*
- Identifying governing bioprocess / mathematical relation:
- In a continuous stirred tank bioreactor (chemostat) at steady state:
$\mu = D = \frac{F}{V}$
where $\mu$ is the specific growth rate ($ ext{h}^{-1}$), $D$ is the dilution rate, $F$ is volumetric feed flow rate, and $V$ is working volume.
$S = \frac{K_s D}{\mu_{max} - D}, \quad X = Y_{X/S}(S_0 - S)$
- **Calculated Quantitative Value**: **10 to 10**.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **10 to 10**
- **Scoring Criteria**: 2 Marks (Negative marking: -0.0 marks).
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