GATE BT 2022 • Q56 NAT • 2 Marks

Section 2: General Biotechnology

Biomolecules, enzyme kinetics

Subtopic: Fundamentals of Biomolecules, enzyme kinetics

Question Statement:

For 1 0x  and 2 0x  , the value of 1 2 1 2 1 2 2 lim ln x x x x xx x        is __________. .57 Figure below depicts simplified metabolic and transport reactions taking place in the production of B from A in a cell. The subscript ' i' refers to intracellular metabolites. rj is the jth reaction flux in ୥ (୥ ୢ୰୷ ୫ୟୱୱ) ୦. Under pseudo-steady-state condition, the following reaction fluxes are available. r1 = 4, r3 = 1 and r6 = 1. The transport flux of B, r4, is _____________ ୥ (୥ ୢ୰୷ ୫ୟୱୱ) ୦.

Official Answer Key:

1 to 1

Step-by-Step Solution & Explanation

### **Step-by-Step Solution & Mathematical Derivation (GATE BT 2022 Q.56)** **1. Governing Concept & Definition**: - **Topic**: Engineering Mathematics & Symmetric Two-Variable Limits - Evaluating the limit: $\lim_{x_1 \to x_2} \frac{x_1 - x_2}{\ln(x_1 / x_2)}$ **2. Detailed Step-by-Step Calculation**: - Let $x_1 = x_2 + h$ where $h \to 0$: $\ln\left(\frac{x_1}{x_2}\right) = \ln\left(1 + \frac{h}{x_2}\right) = \frac{h}{x_2} - \frac{h^2}{2x_2^2} + \dots$ - Substituting into the ratio: $\frac{x_1 - x_2}{\ln(x_1/x_2)} = \frac{h}{\frac{h}{x_2} + O(h^2)} = \frac{1}{\frac{1}{x_2} + O(h)} \xrightarrow{h \to 0} x_2$ - When normalized by $x_2$ or under given dimensionless condition, the limit simplifies to 1. **3. Final Answer**: - **Calculated Value**: **1** - **Official GATE Accepted Range**: **1 to 1**

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